Name: GKE-5008 - AdvanceBio Endo F2, recombinant
Brand: Agilent Technologies
SKU: GKE-5008
Availability: InStock

AdvanceBio Endo F2, recombinant

Specifications

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Enzyme Applications	Selective release of high mannose and biantennary complex type N-linked oligosaccharides from glycopeptides/ glycoproteins.
Enzyme Formulation	10 mM sodium acetate pH 4.5, 25 mM NaCl, 0.02% sodium azide
Enzyme Source	Recombinant gene from Elizabethkingia meningoseptica, expressed in E. coli. The source organism was previously known as Chryseobacterium [Flavobacterium] meningosepticum. Enzyme is also known as Endoglycosidase F2, endo-beta-N-acetylglucosaminidase.
Enzyme Specificity	The enzyme cleaves within the di-N-acetyl chitobiose (GlcNAc-GlcNAc) core of high mannose and biantennary complex type N-linked glycans of oligosaccharide, glycopeptide, or glycoprotein substrates. A single GlcNAc residue remains attached to the molecule after hydrolysis, the rest of the glycan is released. This specificity is distinct from PNGase F (GKE-5006), which cleaves between asparagine and the innermost GlcNAc of N-linked glycans. EndoF2 cleaves between GlcNAc-GlcNac in biantennary glycans at a rate approximately 20 times higher than high mannose glycans. The enzyme is not active towards hybrid type or tri- and tetraantennary oligosaccharides.
Enzyme Unit Definition	One milliunit is defined as the amount of enzyme that will hydrolyze 1 nM of the dansylated porcine fibrinogen glycopeptide substrate DnsVGEN(CHO)R per minute at pH 4.5 and 37°C.
Unit	6 mU
Volume	60 µL
pH Optimum	4.5

GKE-5008 — AdvanceBio Endo F2, recombinant