The XBridge Premier Peptide BEH C18, 300Å, 2.5 µm, 2.1 x 150 mm, with VanGuard FIT Columns have been specially optimized and QC tested with a gradient separation of a tryptic digest of cytochrome c (p/n: 186006371) for peptide separations. The wide-pore (300Å) trifunctionally bonded BEH particle offers the widest usable pH range, superior low pH stability, and ultra-low column bleed to assay especially large peptide samples for proteomics, protein characterization, and peptide synthesis. This column is part of the MaxPeak Premier class of columns, which deliver the chromatographic performance expected from our particle technologies while increasing reproducibility, peak shape, and recovery for metal-sensitive analytes such as phosphorylated peptides. The unique column design includes a fully integrated and easily replaceable 5mm pre-column that serves to protect and extend the life of the full-length analytical column, but adds no dispersion and has no impact on the resolution of your analytical separations. VanGuard FIT Cartridge replacements contain the same qualified sorbent as the analytical column and can easily be replaced.
Suggested Use: Peptide Separations, Peptide Analysis
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Chemistry
C18
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Separation Mode
Reversed Phase
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Particle Substrate
Hybrid
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pH Range Min
1 pH
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pH Range Max
12 pH
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Maximum Pressure
10000 psi (690 Bar)
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Endcapped
Yes
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Silanol Activity
Low
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Particle Shape
Spherical
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Particle Size
2.5 µm
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Endfitting Type
Parker-style
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Pore Size
300 Å
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QC Tested
Peptide
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Format
Premier Column
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Packing Solvent
Acetonitrile
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System
UPLC, UHPLC
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Particle Technology
BEH
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Technique
LC
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USP Classification
L1
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Inner Diameter
2.1 mm
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Length
150 mm
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Carbon Load
18 %
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eCord
Yes
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UNSPSC
41115711
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Application
Peptide
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Brand
XBridge
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Product Type
Columns
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Units per Package
1 pk