<!— "inside defaultSpecification":{Cell Strain=BL21 (DE3)pLysS, Cell Type — Production Method=Chemically Competent, Competent Cell Suitable For=High-level protein expression and easy induction with IPTG and T&, Difficult Protein Expression Type=Toxic Proteins, Generates Unmethylated DNA=No, Genotypes=E. coli B F– ompT hsdS(rB– mB–) dcm+ Tetr gal l(DE3) endA Hte [pLysS Camr], Resistance|MultiLine=Chloramphenicol|Tetracycline|, Transformation Efficiency — Quantitative=1 x 10^8, Volume=10 x 0.1 mL}—>
| Cell Strain |
|
<!— "inside defaultSpecification":{Cell Strain=BL21 (DE3)pLysS, Cell Type — Production Method=Chemically Competent, Competent Cell Suitable For=High-level protein expression and easy induction with IPTG and T&, Difficult Protein Expression Type=Toxic Proteins, Generates Unmethylated DNA=No, Genotypes=E. coli B F– ompT hsdS(rB– mB–) dcm+ Tetr gal l(DE3) endA Hte [pLysS Camr], Resistance|MultiLine=Chloramphenicol|Tetracycline|, Transformation Efficiency — Quantitative=1 x 10^8, Volume=10 x 0.1 mL}—>
| Cell Type — Production Method |
|
<!— "inside defaultSpecification":{Cell Strain=BL21 (DE3)pLysS, Cell Type — Production Method=Chemically Competent, Competent Cell Suitable For=High-level protein expression and easy induction with IPTG and T&, Difficult Protein Expression Type=Toxic Proteins, Generates Unmethylated DNA=No, Genotypes=E. coli B F– ompT hsdS(rB– mB–) dcm+ Tetr gal l(DE3) endA Hte [pLysS Camr], Resistance|MultiLine=Chloramphenicol|Tetracycline|, Transformation Efficiency — Quantitative=1 x 10^8, Volume=10 x 0.1 mL}—>
| Competent Cell Suitable For |
- High-level protein expression and easy induction with IPTG and T&
|
<!— "inside defaultSpecification":{Cell Strain=BL21 (DE3)pLysS, Cell Type — Production Method=Chemically Competent, Competent Cell Suitable For=High-level protein expression and easy induction with IPTG and T&, Difficult Protein Expression Type=Toxic Proteins, Generates Unmethylated DNA=No, Genotypes=E. coli B F– ompT hsdS(rB– mB–) dcm+ Tetr gal l(DE3) endA Hte [pLysS Camr], Resistance|MultiLine=Chloramphenicol|Tetracycline|, Transformation Efficiency — Quantitative=1 x 10^8, Volume=10 x 0.1 mL}—>
| Difficult Protein Expression Type |
|
<!— "inside defaultSpecification":{Cell Strain=BL21 (DE3)pLysS, Cell Type — Production Method=Chemically Competent, Competent Cell Suitable For=High-level protein expression and easy induction with IPTG and T&, Difficult Protein Expression Type=Toxic Proteins, Generates Unmethylated DNA=No, Genotypes=E. coli B F– ompT hsdS(rB– mB–) dcm+ Tetr gal l(DE3) endA Hte [pLysS Camr], Resistance|MultiLine=Chloramphenicol|Tetracycline|, Transformation Efficiency — Quantitative=1 x 10^8, Volume=10 x 0.1 mL}—>
| Generates Unmethylated DNA |
|
<!— "inside defaultSpecification":{Cell Strain=BL21 (DE3)pLysS, Cell Type — Production Method=Chemically Competent, Competent Cell Suitable For=High-level protein expression and easy induction with IPTG and T&, Difficult Protein Expression Type=Toxic Proteins, Generates Unmethylated DNA=No, Genotypes=E. coli B F– ompT hsdS(rB– mB–) dcm+ Tetr gal l(DE3) endA Hte [pLysS Camr], Resistance|MultiLine=Chloramphenicol|Tetracycline|, Transformation Efficiency — Quantitative=1 x 10^8, Volume=10 x 0.1 mL}—>
| Genotypes |
|
<!— "inside defaultSpecification":{Cell Strain=BL21 (DE3)pLysS, Cell Type — Production Method=Chemically Competent, Competent Cell Suitable For=High-level protein expression and easy induction with IPTG and T&, Difficult Protein Expression Type=Toxic Proteins, Generates Unmethylated DNA=No, Genotypes=E. coli B F– ompT hsdS(rB– mB–) dcm+ Tetr gal l(DE3) endA Hte [pLysS Camr], Resistance|MultiLine=Chloramphenicol|Tetracycline|, Transformation Efficiency — Quantitative=1 x 10^8, Volume=10 x 0.1 mL}—>
| Resistance |
- <!— "multilineKeyVal pipe value":Chloramphenicol
- Tetracycline
- —> Chloramphenicol
- Tetracycline
-
|
<!— "inside defaultSpecification":{Cell Strain=BL21 (DE3)pLysS, Cell Type — Production Method=Chemically Competent, Competent Cell Suitable For=High-level protein expression and easy induction with IPTG and T&, Difficult Protein Expression Type=Toxic Proteins, Generates Unmethylated DNA=No, Genotypes=E. coli B F– ompT hsdS(rB– mB–) dcm+ Tetr gal l(DE3) endA Hte [pLysS Camr], Resistance|MultiLine=Chloramphenicol|Tetracycline|, Transformation Efficiency — Quantitative=1 x 10^8, Volume=10 x 0.1 mL}—>
| Transformation Efficiency — Quantitative |
|
<!— "inside defaultSpecification":{Cell Strain=BL21 (DE3)pLysS, Cell Type — Production Method=Chemically Competent, Competent Cell Suitable For=High-level protein expression and easy induction with IPTG and T&, Difficult Protein Expression Type=Toxic Proteins, Generates Unmethylated DNA=No, Genotypes=E. coli B F– ompT hsdS(rB– mB–) dcm+ Tetr gal l(DE3) endA Hte [pLysS Camr], Resistance|MultiLine=Chloramphenicol|Tetracycline|, Transformation Efficiency — Quantitative=1 x 10^8, Volume=10 x 0.1 mL}—>
| Volume |
|
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