<!— "inside defaultSpecification":{Cell Strain=ArcticExpress (DE3)RIL, Cell Type — Production Method=Chemically Competent, Competent Cell Suitable For=Improved protein soluability and codon bias, Difficult Protein Expression Type|MultiLine=Codon Bias|Enhanced Solubility|, Extra Copies of tRNA Genes|MultiLine=argU (AGA, AGG)|ileY (AUA)|leuW (CUA)|, Generates Unmethylated DNA=No, Genotypes=E. coli B F– ompT hsdS(rB– mB–) dcm+ Tetr gal l(DE3) endA Hte [cpn10 cpn60 Gentr] [argU ileY leuW Strr], Resistance=Tetracycline, Transformation Efficiency — Quantitative=5 x 10^6, Volume=10 x 0.1 mL}—>
| Cell Strain |
|
<!— "inside defaultSpecification":{Cell Strain=ArcticExpress (DE3)RIL, Cell Type — Production Method=Chemically Competent, Competent Cell Suitable For=Improved protein soluability and codon bias, Difficult Protein Expression Type|MultiLine=Codon Bias|Enhanced Solubility|, Extra Copies of tRNA Genes|MultiLine=argU (AGA, AGG)|ileY (AUA)|leuW (CUA)|, Generates Unmethylated DNA=No, Genotypes=E. coli B F– ompT hsdS(rB– mB–) dcm+ Tetr gal l(DE3) endA Hte [cpn10 cpn60 Gentr] [argU ileY leuW Strr], Resistance=Tetracycline, Transformation Efficiency — Quantitative=5 x 10^6, Volume=10 x 0.1 mL}—>
| Cell Type — Production Method |
|
<!— "inside defaultSpecification":{Cell Strain=ArcticExpress (DE3)RIL, Cell Type — Production Method=Chemically Competent, Competent Cell Suitable For=Improved protein soluability and codon bias, Difficult Protein Expression Type|MultiLine=Codon Bias|Enhanced Solubility|, Extra Copies of tRNA Genes|MultiLine=argU (AGA, AGG)|ileY (AUA)|leuW (CUA)|, Generates Unmethylated DNA=No, Genotypes=E. coli B F– ompT hsdS(rB– mB–) dcm+ Tetr gal l(DE3) endA Hte [cpn10 cpn60 Gentr] [argU ileY leuW Strr], Resistance=Tetracycline, Transformation Efficiency — Quantitative=5 x 10^6, Volume=10 x 0.1 mL}—>
| Competent Cell Suitable For |
- Improved protein soluability and codon bias
|
<!— "inside defaultSpecification":{Cell Strain=ArcticExpress (DE3)RIL, Cell Type — Production Method=Chemically Competent, Competent Cell Suitable For=Improved protein soluability and codon bias, Difficult Protein Expression Type|MultiLine=Codon Bias|Enhanced Solubility|, Extra Copies of tRNA Genes|MultiLine=argU (AGA, AGG)|ileY (AUA)|leuW (CUA)|, Generates Unmethylated DNA=No, Genotypes=E. coli B F– ompT hsdS(rB– mB–) dcm+ Tetr gal l(DE3) endA Hte [cpn10 cpn60 Gentr] [argU ileY leuW Strr], Resistance=Tetracycline, Transformation Efficiency — Quantitative=5 x 10^6, Volume=10 x 0.1 mL}—>
| Difficult Protein Expression Type |
- <!— "multilineKeyVal pipe value":Codon Bias
- Enhanced Solubility
- —> Codon Bias
- Enhanced Solubility
-
|
<!— "inside defaultSpecification":{Cell Strain=ArcticExpress (DE3)RIL, Cell Type — Production Method=Chemically Competent, Competent Cell Suitable For=Improved protein soluability and codon bias, Difficult Protein Expression Type|MultiLine=Codon Bias|Enhanced Solubility|, Extra Copies of tRNA Genes|MultiLine=argU (AGA, AGG)|ileY (AUA)|leuW (CUA)|, Generates Unmethylated DNA=No, Genotypes=E. coli B F– ompT hsdS(rB– mB–) dcm+ Tetr gal l(DE3) endA Hte [cpn10 cpn60 Gentr] [argU ileY leuW Strr], Resistance=Tetracycline, Transformation Efficiency — Quantitative=5 x 10^6, Volume=10 x 0.1 mL}—>
| Extra Copies of tRNA Genes |
- <!— "multilineKeyVal pipe value":argU (AGA, AGG)
- ileY (AUA)
- leuW (CUA)
- —> argU (AGA, AGG)
- ileY (AUA)
- leuW (CUA)
-
|
<!— "inside defaultSpecification":{Cell Strain=ArcticExpress (DE3)RIL, Cell Type — Production Method=Chemically Competent, Competent Cell Suitable For=Improved protein soluability and codon bias, Difficult Protein Expression Type|MultiLine=Codon Bias|Enhanced Solubility|, Extra Copies of tRNA Genes|MultiLine=argU (AGA, AGG)|ileY (AUA)|leuW (CUA)|, Generates Unmethylated DNA=No, Genotypes=E. coli B F– ompT hsdS(rB– mB–) dcm+ Tetr gal l(DE3) endA Hte [cpn10 cpn60 Gentr] [argU ileY leuW Strr], Resistance=Tetracycline, Transformation Efficiency — Quantitative=5 x 10^6, Volume=10 x 0.1 mL}—>
| Generates Unmethylated DNA |
|
<!— "inside defaultSpecification":{Cell Strain=ArcticExpress (DE3)RIL, Cell Type — Production Method=Chemically Competent, Competent Cell Suitable For=Improved protein soluability and codon bias, Difficult Protein Expression Type|MultiLine=Codon Bias|Enhanced Solubility|, Extra Copies of tRNA Genes|MultiLine=argU (AGA, AGG)|ileY (AUA)|leuW (CUA)|, Generates Unmethylated DNA=No, Genotypes=E. coli B F– ompT hsdS(rB– mB–) dcm+ Tetr gal l(DE3) endA Hte [cpn10 cpn60 Gentr] [argU ileY leuW Strr], Resistance=Tetracycline, Transformation Efficiency — Quantitative=5 x 10^6, Volume=10 x 0.1 mL}—>
| Genotypes |
- E. coli B F– ompT hsdS(rB– mB–) dcm+ Tetr gal l(DE3) endA Hte [cpn10 cpn60 Gentr] [argU ileY leuW Strr]
<!— Genotypes::E. coli B F– ompT hsdS(rB– mB–) dcm+ Tetr gal l(DE3) endA Hte [cpn10 cpn60 Gentr] [argU ileY leuW Strr] —>
|
<!— "inside defaultSpecification":{Cell Strain=ArcticExpress (DE3)RIL, Cell Type — Production Method=Chemically Competent, Competent Cell Suitable For=Improved protein soluability and codon bias, Difficult Protein Expression Type|MultiLine=Codon Bias|Enhanced Solubility|, Extra Copies of tRNA Genes|MultiLine=argU (AGA, AGG)|ileY (AUA)|leuW (CUA)|, Generates Unmethylated DNA=No, Genotypes=E. coli B F– ompT hsdS(rB– mB–) dcm+ Tetr gal l(DE3) endA Hte [cpn10 cpn60 Gentr] [argU ileY leuW Strr], Resistance=Tetracycline, Transformation Efficiency — Quantitative=5 x 10^6, Volume=10 x 0.1 mL}—>
| Resistance |
|
<!— "inside defaultSpecification":{Cell Strain=ArcticExpress (DE3)RIL, Cell Type — Production Method=Chemically Competent, Competent Cell Suitable For=Improved protein soluability and codon bias, Difficult Protein Expression Type|MultiLine=Codon Bias|Enhanced Solubility|, Extra Copies of tRNA Genes|MultiLine=argU (AGA, AGG)|ileY (AUA)|leuW (CUA)|, Generates Unmethylated DNA=No, Genotypes=E. coli B F– ompT hsdS(rB– mB–) dcm+ Tetr gal l(DE3) endA Hte [cpn10 cpn60 Gentr] [argU ileY leuW Strr], Resistance=Tetracycline, Transformation Efficiency — Quantitative=5 x 10^6, Volume=10 x 0.1 mL}—>
| Transformation Efficiency — Quantitative |
|
<!— "inside defaultSpecification":{Cell Strain=ArcticExpress (DE3)RIL, Cell Type — Production Method=Chemically Competent, Competent Cell Suitable For=Improved protein soluability and codon bias, Difficult Protein Expression Type|MultiLine=Codon Bias|Enhanced Solubility|, Extra Copies of tRNA Genes|MultiLine=argU (AGA, AGG)|ileY (AUA)|leuW (CUA)|, Generates Unmethylated DNA=No, Genotypes=E. coli B F– ompT hsdS(rB– mB–) dcm+ Tetr gal l(DE3) endA Hte [cpn10 cpn60 Gentr] [argU ileY leuW Strr], Resistance=Tetracycline, Transformation Efficiency — Quantitative=5 x 10^6, Volume=10 x 0.1 mL}—>
| Volume |
|
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—>
