600191

Name: 600191
Brand: Agilent Technologies
SKU: 600191
Availability: InStock

Бренд: Agilent Technologies

Specifications

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Concentration	4 U/µL
Inactivation	Must be removed by protein extraction methods
Ligase Type	Pfu DNA ligase
Quantity per Kit	400 U
Reaction Conditions	4 U of Pfu DNA ligase enzyme, in 20 mM Tris-HCl (pH 7.5), 20 mM KCl, 10 mM MgCl2, 0.1 % Igepal, 0.01 mM rATP, 1 mM DTT
Reactions per Kit	400 units
Storage Buffer	50 mM Tris-HCl (pH 7.5), 50 % glycerol, 1 mM EDTA, 10 mM DTT, 0.1 % Tween 20, 0.1 % Igepal
Unit Definition	One unit will ligate to completion 0.5 µg of nicked pBluescript DNA in 15 minutes at 55°C.