AAV-293 Cells

AAV-293 cells are an HEK293 cell line optimized for packaging of AAV virions in the helper-free system. AAV-293 cells, like HEK293 cells, produce E1 in trans. AAV-293 cells allow production of infectious virus particles when transfected with E1-deleted adenovirus vectors or co-transfected with the three AAV helper-free system plasmids. These plasmids supply all of the trans-acting factors required for AAV replication and packaging in AAV-293 cells. Recombinant AAV-2 viral particles prepared from infected AAV-293 cells may then be used to infect a variety of mammalian cells.