ACQUITY Premier Protein SEC 250 Å, 1.7 µm Columns help scientists obtain reliable protein size variant separations (10,000 to approx 650,000 Daltons) via the synergistic coupling of Waters MaxPeak Premier High Performance Surfaces and novel BEH-PEO Particle technologies. These advancements in SEC column hardware and SEC particle technology work to minimize non-desired secondary ionic or hydrophobic interactions between proteins and the column to minimize costly method development work by allowing chromatographers to use a “generic” or “platform-type” method for many samples. Note: The same column is also available with the Waters mAb Size Variant Standard at no additional cost. (p/n: 176005070) Also note that for optimal SEC separation performance, this column is designed for use in UPLC SEC applications with an appropriate low LC system volume.
-
Chemistry
SEC
-
Separation Mode
SEC
-
Particle Substrate
Hybrid
-
pH Range Min
2.5 pH
-
pH Range Max
8 pH
-
Temperature Limits
60 C
-
Maximum Pressure
6000 psi (415 Bar)
-
Endcapped
No
-
Silanol Activity
Low
-
Molecular Weight Range Min
10000
-
Molecular Weight Range Max
650,000
-
Particle Size
1.7 µm
-
Endfitting Type
Parker-style
-
Pore Size
250 Å
-
QC Tested
Protein
-
Format
Premier Column
-
Surface Area
174
-
System
UHPLC, UPLC
-
Particle Technology
BEH
-
Technique
LC
-
USP Classification
L33, L59
-
Inner Diameter
4.6 mm
-
Length
100 mm
-
Carbon Load
12 %
-
UNSPSC
41115709
-
Application
Protein
-
Brand
ACQUITY
-
Product Type
Columns
-
Units per Package
1 pk