ACQUITY Premier Protein SEC Column, 250 Å, 1.7 µm, 4.6 x 300 mm Columns help scientists obtain reliable protein size variant separations (10,000 to approx 650,000 Daltons) via the synergistic coupling of Waters MaxPeak Premier High Performance Surfaces and novel BEH-PEO Particle technologies. These advancements in SEC column hardware and SEC particle technology work to minimize non-desired secondary ionic or hydrophobic interactions between proteins and the column to minimize costly method development work by allowing chromatographers to use a «generic» or «platform-type» method for many samples. Each batch is specifically QC tested using Waters mAb Size Variant Standard (p/n: 186009429) to help ensure consistent performance for challenging size-based protein separations.
Note: The same column is also available with the Waters mAb Size Variant Standard at no additional cost. (p/n: 176005072) Also note that for optimal SEC separation performance, this column is designed for use in UPLC SEC applications with an appropriate low LC system volume.
Suggested Use: Gel Filtration, Size Exclusion Chromatography, Aggregate, Monomer and Fragment analysis, Antibody Conjugates
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Chemistry
SEC
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Separation Mode
SEC
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Particle Substrate
Hybrid
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pH Range Min
2.5 pH
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pH Range Max
8 pH
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Temperature Limits
60 C
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Maximum Pressure
11000 PSI (759 bar)
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Endcapped
No
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Bonding Technology
BEH-PEO
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Silanol Activity
Low
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Molecular Weight Range Min
10000
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Molecular Weight Range Max
650,000
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Particle Size
1.7 µm
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Endfitting Type
Parker-style
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Pore Size
250 Å
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QC Tested
Protein
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Format
Online Column
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Surface Area
174
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System
UHPLC, UPLC, HPLC
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Particle Technology
BEH
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Technique
LC
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USP Classification
L33, L59
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Inner Diameter
4.6 mm
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Length
300 mm
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Carbon Load
12 %
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eCord
Yes
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UNSPSC
41115709
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Application
Protein
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Brand
ACQUITY
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Product Type
Columns
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Units per Package
1 pk