ACQUITY Premier Protein SEC 250Å, 1.7 µm Columns help scientists obtain reliable protein size variant separations (10,000 to approx 650,000 Daltons) via the synergistic coupling of Waters MaxPeak Premier High Performance Surfaces and novel BEH-PEO Particle technologies. These advancements in SEC column hardware and SEC particle technology work to minimize non-desired secondary ionic or hydrophobic interactions between proteins and the column to minimize costly method development work by allowing chromatographers to use a “generic” or “platform-type” method for many samples. Note: This column includes the recommended MaxPeak Premier SEC 250Å, 2.5 µm, 4.6 x 30 mm Guard (p/n: 186009969) to help extend column life without sacrifycing desired component resolution. Also note that for optimal SEC separation performance, this column is designed for use in UPLC SEC applications with an appropriate low LC system volume.
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Chemistry
SEC
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Separation Mode
SEC
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Particle Substrate
Hybrid
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pH Range Min
2.5 pH
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pH Range Max
8 pH
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Temperature Limits
60 C
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Maximum Pressure
11000 PSI (759 bar)
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Endcapped
No
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Bonding Technology
BEH-PEO
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Silanol Activity
Low
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Molecular Weight Range Min
10000
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Molecular Weight Range Max
650,000
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Particle Size
1.7 µm
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Endfitting Type
Parker-style
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Pore Size
250 Å
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QC Tested
Protein
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Format
Online Column
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Surface Area
174
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System
UPLC
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Particle Technology
BEH
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Technique
LC
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USP Classification
L33, L59
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Inner Diameter
4.6 mm
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Length
300 mm
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Carbon Load
12 %
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eCord
Yes
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UNSPSC
41115709
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Application
Protein
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Brand
ACQUITY
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Product Type
Columns
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Units per Package
1 pk