ACQUITY UPLC M-Class Columns are designed for optimal performance for nano- to micro scale liquid chromatographic separation, offering the highest possible sensitivity, resolution and reproducibility for biomarker discovery and protein identification and characterization. As the 75µm to 180µm ID columns are fritted with a novel frit technology, they are able to withstand 15kpsi operating pressure and handle pressure fluctuations during injection extremely well and thus provide superior longevity. In order to achieve maximum performance, it is important to ensure that proper connections are made to minimize peak tailing and poor efficiency. This specific offering contains Waters patented BEH Technology 130A pore, C18, 1.7um particles. Each batch is specifically QC tested using a gradient separation of a tryptic digest of cytochrome c to before being QC accepted as a Peptide separation column.
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Chemistry
C18
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Separation Mode
Reversed Phase
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Particle Substrate
Hybrid
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pH Range Min
1 pH
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pH Range Max
10 pH
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Maximum Pressure
15000 psi (1034 Bar)
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Endcapped
Yes
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Silanol Activity
Low
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Particle Shape
Spherical
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Particle Size
1.7 µm
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Endfitting Type
Valco(male)|360um Open
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Pore Size
130 Å
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QC Tested
Peptide
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Format
Column
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Surface Area
185
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System
Nano/Micro
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Particle Technology
BEH
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USP Classification
L1
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Inner Diameter
75 µm
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Length
100 mm
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Carbon Load
18 %
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UNSPSC
41115709
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Application
Peptide
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Brand
ACQUITY UPLC M-Class
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Product Type
Columns
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Units per Package
1 pk