ACQUITY UPLC M-Class Columns are designed for optimal performance for nano- to micro scale liquid chromatographic separation for biomarker discovery and protein and peptide identification and characterization. All M-Class columns are designed for 15 kpsi (1,000 bar) operating pressure and handle pressure fluctuations during injection extremely well and thus provide superior longevity.
This specific offering contains Waters patented BEH Technology 300 Å pore, C18, 1.7um particles. BEH technology offers the highest retentivity for basic compounds and provides excellent peak shape at elevated pH mobile phases and is suitable for separations up to 80°C. BEH is the universal column choice for a variety of compounds.
Each batch is specifically QC tested using a gradient separation of a tryptic digest of cytochrome c to before being QC accepted as a Peptide Separation Column.
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Chemistry
C18
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Separation Mode
Reversed Phase
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Particle Substrate
Hybrid
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pH Range Min
1 pH
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pH Range Max
10 pH
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Maximum Pressure
15000 psi (1034 Bar)
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Endcapped
Yes
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Silanol Activity
Low
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Particle Shape
Spherical
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Particle Size
1.7 µm
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Endfitting Type
Valco(male)|360um Open
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Pore Size
300 Å
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QC Tested
Peptide
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Format
Column
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Surface Area
90
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System
Nano/Micro
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Particle Technology
BEH
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USP Classification
L1
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Inner Diameter
75 µm
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Length
250 mm
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Carbon Load
12 %
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UNSPSC
41115709
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Application
Peptide
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Brand
ACQUITY UPLC M-Class
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Product Type
Columns
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Units per Package
1 pk