Waters Protein Separation Technology (PrST) columns are QC tested and optimized to separate proteins based on size, hydrophobicity and isoelectric point. If you compare to traditional C18 phases, the C4 ligand is less retentive and will minimize protein carryover, increase protein recovery, and improve peak capacity. The VanGuard pre-column connects directly to the inlet of the ACQUITY UPLC column which minimizes extra columns volumes and reduces leaking by eliminating additional connections.
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Chemistry
C4
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Separation Mode
Reversed Phase
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Particle Substrate
Hybrid
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pH Range Min
1 pH
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pH Range Max
12 pH
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Maximum Pressure
18000 psi (1240 Bar)
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Endcapped
No
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Silanol Activity
Low
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Molecular Weight Range Min
10000
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Molecular Weight Range Max
500000
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Particle Shape
Spherical
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Particle Size
1.7 µm
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Endfitting Type
Direct-Connect
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Pore Size
300 Å
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QC Tested
Protein
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Format
VanGuard Pre-column
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System
UPLC, UHPLC
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Particle Technology
BEH
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USP Classification
L26
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Inner Diameter
2.1 mm
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Length
5 mm
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Carbon Load
8 %
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UNSPSC
41115709
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Application
Protein
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Brand
ACQUITY UPLC
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Product Type
Guards
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Units per Package
3 pk