Based upon the highly successful BEH particle technology, the ACQUITY UPLC Protein BEH SEC Column, 200 Å, 1.7 µm, 4.6 mm x 150 mm represents the first commercially available sub-2-µm SEC chemistry. Fully optimized for the analysis of proteins and their aggregates with molecular weights ranging from 10000 to 450,000 Daltons, the total system solution will routinely deliver fast, accurate aggregate determinations up to 10 times quicker that traditional HPLC-based SEC methods. Note that for optimal SEC separation performance, this column is designed for use in UPLC SEC applications with an appropriate low LC system volume.
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Chemistry
SEC
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Separation Mode
SEC
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Particle Substrate
Hybrid
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pH Range Min
2.5 pH
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pH Range Max
8 pH
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Temperature Limits
60 C
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Maximum Pressure
7000 psi (483 Bar)
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Endcapped
No
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Bonding Technology
Diol (OH)
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Silanol Activity
Low
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Molecular Weight Range Min
10000
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Molecular Weight Range Max
450000
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Particle Shape
Spherical
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Particle Size
1.7 µm
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Endfitting Type
Parker-style
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Pore Size
200 Å
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QC Tested
Protein
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Format
Column
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Packing Solvent
20% methanol in water
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Surface Area
220
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System
UPLC, UHPLC
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Particle Technology
BEH
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Technique
SEC
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USP Classification
L33
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Inner Diameter
4.6 mm
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Length
150 mm
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Carbon Load
12 %
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eCord
Yes
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UNSPSC
41115709
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Application
Protein
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Brand
ACQUITY UPLC
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Product Type
Columns
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Units per Package
1 pk