Based upon the highly successful BEH particle technology, the ACQUITY UPLC Protein BEH SEC Column, 450 Å, 2.5 µm, 4.6 mm x 150 mm is fully optimized for the analysis of proteins and their aggregates with molecular weights ranging from 100,000 to 1,500,000 Daltons, the total system solution will routinely deliver fast, accurate aggregate determinations up to 10 times quicker that traditional HPLC-based SEC methods.
Note: This column is also shipped with the BEH450 SEC Protein Standardat no additional cost at no additional cost (PN: 176002996) . Also note that for optimal SEC separation performance, this column is designed for use in UPLC SEC applications with an appropriate low LC system volume.
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Chemistry
SEC
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Separation Mode
SEC/GPC
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Particle Substrate
Hybrid
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pH Range Min
2.5 pH
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pH Range Max
8 pH
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Temperature Limits
60 C
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Maximum Pressure
3000 psi (207 Bar)
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Endcapped
No
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Bonding Technology
SEC
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Silanol Activity
Low
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Molecular Weight Range Min
100000
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Molecular Weight Range Max
1500000
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Particle Shape
Spherical
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Particle Size
2.5 µm
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Endfitting Type
Parker-style
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Pore Size
450 Å
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QC Tested
Protein
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Format
Column
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Packing Solvent
20% methanol in water
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Surface Area
80
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System
UPLC
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Particle Technology
BEH
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Technique
LC
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USP Classification
L33
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Inner Diameter
4.6 mm
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Length
150 mm
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Carbon Load
9 %
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eCord
Yes
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UNSPSC
41115709
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Application
Protein
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Brand
ACQUITY UPLC
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Product Type
Columns
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Units per Package
1 pk