BCL2 IQFISH Break-Apart Probe

BCL2 IQFISH Break-Apart Probe is intended for the detection of rearrangements involving the BCL2 gene by fluorescence in situ hybridization (FISH). The probe is to be used on formalin-fixed, paraffin-embedded tissue sections.Break apart probes consist of two child probes (3’ and 5’ probes), designed to hybridize on opposite sides of the translocation break point for a given gene, each labeled in a different color (Cy3 and FITC). These probes generate signals in normal cells that are closely matched in size and co-localized (2 fusion signals). Following a translocation, the signals are “broken apart” and no longer co-localize (for example: 1 orange-red, 1 green, 1 fusion). BCL2 IQFISH Break-Apart Probe and corresponding protocol were developed using formalin-fixed, paraffin-embedded, lymph node tissue sections.Child Probe 5′ RD, Chr18; Start 61043686, Stop 61641631Child Probe 3′ GR, Chr18; Start 60140026, Stop 60763791