bioZen 2.6 µm WidePore C4, LC Column 250 x 4.6 mm, Ea
Бренд: Phenomenex
Minimize your priming with our BioTi Biocompatible hardware
bioZen Titanium BioTi Hardware
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Columns:
bioZen 1.8 μm SEC-3
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Dimension:
150 x 4.6 mm
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Mobile Phase:
50 mM Dipotassium Phosphate + 100 mM Sodium Sulfate, pH 5.0
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Flow Rate:
0.3 mL/min
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Temperature:
Ambient
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Detection:
UV @ 280 nm
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Sample:
1. γ-Globulin, 5 mg/mL 2. Ovalbumin, 1 mg/mL
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Diverse mAb Comparison with Chromatographic Performance Suitable for Intact MS Analysis
- High throughput and single method for multiple mAb analysis
- High temperature leads improved peak shape for all mAbs
- Formic acid mobile phase ensures sensitivity for intact mass by high resolution MS
LC Conditions
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Columns:
bioZen 2.6 µm WidePore C4
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Dimension:
100 x 2.1 mm
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Part No.:
00D-4786-AN
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Mobile Phase:
A: 0.1 % Formic acic in Water B: 0.1 % Formic acic in Acetonitrile
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Gradient:
10-90 % B in 4 minutes
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Flow Rate:
0.3 mL/min
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Temperature:
80 °C
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Detection:
UV @ 280 nm
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Sample:
mAbs, Various (1 mg/mL)
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Improved peak shape for intact mass applications by high resolution MS.
| Sample | Retention Time (min) | Width @ 50% |
|---|---|---|
| Rituximab | 3.580 | 0.0233 |
| Infliximab Biosimilar | 3.606 | 0.0272 |
| Cetuximab | 3.696 | 0.0270 |
| Infliximab | 3.615 | 0.0222 |
Temperature Effects on Intact and Subunit Analysis
- Displays improved temperatures for Intact Influximab and Reduced NIST mAb at 70, 80, and 90 ºC
- 80 ºC exhibits narrowest and most ideal peak shape for both the intact and subunit analysis
LC Conditions
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Columns:
bioZen 2.6 µm WidePore C4
|
|
Dimension:
100 x 2.1 mm
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|
Part No.:
00D-4786-AN
|
|
Mobile Phase:
A: 0.1 % Formic acic in Water B: 0.1 % Formic acic in Acetonitrile
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|
Gradient:
10-90 % B in 4 minutes
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|
Flow Rate:
0.3 mL/min
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|
Temperature:
80 °C
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|
Detection:
UV @ 280 nm
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Sample:
mAbs, Various (1 mg/mL)
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