bioZen 3.6 µm Intact XB-C8, LC Column 100 x 2.1 mm, Ea
Бренд: Phenomenex
Minimize your priming with our BioTi™ Biocompatible hardware
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Columns:
bioZen 1.8 μm SEC-3
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Dimension:
150 x 4.6 mm
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Mobile Phase:
50 mM Dipotassium Phosphate + 100 mM Sodium Sulfate, pH 5.0
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Flow Rate:
0.3 mL/min
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Temperature:
Ambient
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Detection:
UV @ 280 nm
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Sample:
1. γ-Globulin, 5 mg/mL 2. Ovalbumin, 1 mg/mL
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Intact and Fragment Analysis
Impurity profiling and characterization of intact biologic fragments is a challenging undertaking because of the need to identify very small differences between variants. Both bioZen Intact columns contain skillfully manufactured large pore core-shell particles that provide narrower, taller peaks in conjunction with higher resolution between the target HC/LC, Fc/Fab, or isoforms.
Intact Trastuzumab at 70, 80, and 90°C
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Column:
bioZen 3.6 µm Intact XB-C8
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Dimension:
00F-4766-AN
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Part No.:
150 x 2.1 mm
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Mobile Phase:
A: 0.1 % TFA in Water B: 0.1 % TFA in Acetonitrile
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Gradient:
Time (min)
0 1 13
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% B
20 20 45
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Flow Rate:
0.5 mL/min
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Temperature:
70 °C 80 °C 90 °C
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Detection:
UV @ 280 nm
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Sample:
1. Trastuzumab
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Infliximab F(ab)2
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Column:
bioZen 3.6 µm Intact XB-C8
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Dimension:
150 x 2.1 mm
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Part No.:
00F-4766-AN
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Mobile Phase:
A: 0.1 % TFA in Water B: 0.1 % TFA in Acetonitrile
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Gradient:
Time (min)
0 1 1
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