bioZen 6µm WCX, LC Column 250 x 4.6 mm, Ea
Бренд: Phenomenex
bioZen WCX Monosized Polymeric Non-Porous Particle
Monosized particles grafted with linear polycarboxylate chains to envelop and separate proteins from acidic/basic variants
Minimize your priming and promote better recovery with our BioTi™ biocompatible hardware
Monosized Particles for Optimal Consistency
Our specially crafted polymeric particle is produced with outstanding precision so that you can be confident in increased performance and minimized variation between lots.
Comparative separations may not be representative of all applications.
| Columns: |
| bioZen 6 µm WCX |
| Dimension: |
| 250 x 4.6 mm |
| Part No.: |
| 00G-4777-EO |
| Mobile Phase: |
| A: CX-1 Gradient Buffer A (pH 5.6) B: CX-1 Gradient Buffer B (pH 10.2) |
| Gradient: |
| 0-100 % B in 20 minutes |
| Flow Rate: |
| 1 mL/min |
| Temperature: |
| 30 °C |
| Detection: |
| UV @ 280 nm |
| Sample: |
| Trastuzumab |
| Note: |
| CX-1 Gradient Buffers available from Thermo Fisher Scientific™ |
Charge Variant Analysis
bioZen WCX was crafted to consistently decipher between native protein variants that arise from PTMs within a therapeutics creation and development. The linear polycarboxylate chains grafted to monosized non-porous polymeric particles, envelop and separate proteins from acidic and basic protein variants. With such a highly. tuned and controlled manufacturing process, bioZen WCX media affords scientists a way to reproducibly characterize heterogeneity while taking advantage of excellent recovery through high particle inertness and bioinert titanium BioTi™ column hardware.
Trastuzumab (MES Salt Gradient)
| Column: | |
| bioZen 6 μm WCX | |
| Dimension: | |
| 250 x 4.6 mm | |
| Part No.: | |
| 00G-4777-E0 | |
| Mobile Phase: | |
| A: 20 mM MES (pH 5.6) B: 20 mM MES + 300 mM NaCl (pH 5.6) | |
| Time (min) | % B |
| 0 1 31 31.1 34 35 | 15 15 45 100 100 15 |
| Flow Rate: | |
| 1 mL/min | |
| Temperature: | |
| 30 °C | |
| Detection: | |
| UV @ 280 nm | |
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