Name: Clarity 2.6 µm Oligo-MS 100A, LC Column 50 x 2.1 mm, Ea
Brand: Phenomenex
SKU: 00B-4479-AN
Availability: InStock

Core-Shell Technology for Synthetic DNA/RNA Analysis

Clarity Oligo-MS media is not fully porous like traditional particles used for the analysis of oligonucleotides. It is a core-shell particle technology which uses a sol-gel processing technique to grow a homogeneous porous shell onto a solid core. This highly optimized process combined with uniform particle size distribution produces a column that generates extremely high plate counts.

1.7 µm Core-Shell Particle

Reduced diffusion path maximizes efficiency
Increased efficiencies compared to traditional fully porous sub-2µm columns. Typical operating backpressures > 400 bar

2.6 µm Core-Shell Particle

Reduced diffusion path maximizes efficiency
Ultra-high performance on any system with Clarity Oligo-MS 2.6 µm columns

Achieve Baseline Resolution of N-1 and N+1 Oligo from Target

The high plate counts generated by the Clarity Oligo-MS material produce extremely high efficiencies and thus excellent resolution between oligonucleotides of similar length and structure. Scientists can achieve baseline resolution between synthetic oligonucleotides with just one base difference allowing easier quantitation.

Poly dT Standard (12-18mer)

Column:

Clarity 2.6 µm Oligo-MS C18

Dimension:

50 x 2.1 mm

Part No.:

00B-4479-AN

Mobile Phase:

A: 100 mM HFIP / 4 mM TEA / 2 % Methanol B: 100 mM HFIP / 4 mM TEA / 98 % Methanol

Gradient:

A/B (95:5) to A/B (80:20) in 10 min

Flow Rate:

0.5 mL/ min

Temperature:

50 ˚C

Detection:

UV @ 260 nm (22 ˚C)

Injection Volume:

20 µL

Sample Volume:

Poly dT (12-18)

Rapid Separation of Complex Oligo Samples

Due to the high resolving power of Clarity Oligo-MS columns, high-throughput methods for the separation of complex synthetic mixtures can be developed. Using short (50 mm length) columns, impurities are separated from the peak of interest in less than 12 minutes

Crude DNA 30mer