Name: Clarity 30 µm QSP, 50 mg / well, 96-Well Plates, 40/Pk
Brand: Phenomenex
SKU: 8E-S102-DGS
Availability: InStock

General Extraction

Formats
Oligo Chemistry	96-Well Plates and 3 mL Cartridges
Synthesis Scale Load	DNA & RNA
Oligo Length	50 mg/well 96-well plates and 60 mg/3 mL tubes=up to 200 nmol 150 mg/ 3 ml tubes= up to 1 µmol 5 g/60 mL tubes= up to 25 µmol
Typical Purity¹	≤ 100 nt
Typical Recovery Yield²	≥ 90 %
Typical Recovery Yield²	≥ 80 %
Purification Time	~ 8 minutes/ cartridge ~ 45 minutes/ well plate (96 samples)
Equipment Required	Positive pressure manifold, vacuum manifold, or automated liquid handling system

¹ Purity value based on ion-exchange chromatography and capillary electrophoresis ² OD260 used for quantitation

It’s Quick, Simple, and Pure (QSP)

Trityl-on oligo sample preparation. Mix equal volume of loading buffer with cleavage/deprotection solution

Crude 30 mer DNA- 200 nmole Sequence: GTGGATCTGCGCACTTCAGGCTCCTGGGCG

Sample Preparation:

250 µL of oligo solution in concentrated NH₂OH (post deprotection) was aliquoted
An equal volume of DNA buffer (250 µL) was added and vortexed

Condition:

1 mL Methanol (2 x 0.5 mL)

Equilibrate:

1 mL Water (2 x 0.5 mL)

Load:

Oligo Sample (500 µL)

Detritylate:

1 mL 1 % DCA

Rinse:

1 mL Water (2 x 0.5 mL)

Dry Sorbent:

at 10” Hg Vacuum (~1 min)

Elute:

1 mL 15 mM Na2CO3/ 20 % Acetonitrile

Crude Trityl-on	Load Fraction	Detritylated Final Elution	Recovery	Purity (Peak area)
28.3	5.3	20.8	90.3 %	92 %