Clarity 5 µm Oligo-RP, LC Column 250 x 4.6 mm, Ea
Бренд: Phenomenex
Analytical LC
Clarity Oligo-RP™
Clarity Oligo-RP columns have been specifically designed for the reversed phase purification of oligonucleotides with balanced hydrophobicity and polar selectivity. Available in 3, 5, and 10 µm particles, Clarity Oligo-RP allows for high capacity analysis and purification of oligos.
A Highly Selective Analysis
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Column:
Clarity 3 µm Oligo-RP C18
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Dimension:
50 x 4.6 mm
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Part No.:
00B-4441-E0
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Mobile Phase:
A: 50 mM TEAA pH 7.5 B: Methanol
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Gradient:
10 % to 45 % B in 30 minutes
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Flow Rate:
1 mL/min
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Detection:
UV @ 260 nm
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Sample:
1. 40nt DNA with sequence CTTCTGAACAGTT-GATCTATG-CACTTCAGACTTATGATCA (2.5 μg)
2. 39nt DNA with sequence TTCTGAACAGTTGATCTAT-GCACTTCAGACTTATGATCA (2.5 μg)
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Clarity Oligo-RP successfully separates a 40mer from a 39mer DNA oligonucleotide due to its excellent efficiency and resolving power.
Purification Clarity™
Clarity Oligo-RP columns allow for high loadability and deliver high recovery and purity, from HPLC to Preparative Purification.
Easily Scale from HPLC to Preparative Purification.
DNA Purification
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Column:
Clarity 3 µm Oligo-RP C18
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Dimension:
(A) 50 x 10.0 mm (B) 50 x 4.6 mm
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Mobile Phase:
A: 50 mM TEAA pH 7.5/ 5 % Acetonitrile B: Methanol
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Gradient:
10 % to 60 % B in 20 minutes
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Flow Rate:
(A) 4.7 mL/ min
(B) 1.0 mL/ min
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Detection:
UV @ 280 nm
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Sample:
20nt DNA
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