Clarity RP Desalting, 200 mg / 3 mL, Tubes, 50/Pk
Бренд: Phenomenex
General Extraction
Desalting of Dye-Labeled DNA
A quencher-labeled sample of DNA (25nt) with the sequence FAMTTGACTTAGACTTAGA-CTTAGTTT was desalted using Clarity RP Desalting tubes in the 200 mg/3 mL format. Collection fractions were then analyzed for purity and recovery.
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Column:
Clarity 3 µm Oligo-RP C18
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Dimension:
50 x 4.6 mm
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Part No.:
00B-4441-E0
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Mobile Phase:
A: 50 mM TEAA, pH 7.5 / 5 % Acetonitrile B: Methanol
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Gradient:
A/B (90:10) to A/B (40:60) in 20 min
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Flow Rate:
1 mL/ min
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Detection:
UV @ 260 nm
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Sample:
25nt DNA oligonucleotide
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Column:
Clarity 3 µm Oligo-RP C18
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|
Dimension:
50 x 4.6 mm
|
|
Part No.:
00B-4441-E0
|
|
Mobile Phase:
A: 50 mM TEAA, pH 7.5 / 5 % Acetonitrile B: Methanol
|
|
Gradient:
A/B (90:10) to A/B (40:60) in 20 min
|
|
Flow Rate:
1 mL/ min
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|
Detection:
UV @ 260 nm
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Sample:
40nt DNA
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