Codon Bias

BL21-CodonPlus strains are engineered to contain extra copies of genes that encode the tRNAs most frequently limiting translation of heterologous proteins in E. coli. These host strains are ideal for difficult protein expression, especially when codon bias is an issue.The lambda CE6 induction kit for toxic protein expression handles the challenge of achieving expression of genes under control of the T7 promoter. The lambda CE6 induction kit delivers T7 RNA polymerase into BL21 competent cells to induce expression of toxic proteins from T7 promoter-driven vectors.