Cotton is the world’s most significant textile and oilseed crop and Gossypium hirsutum is the most widely cultivated cotton. Genetic improvement of cotton fiber production for increased yield & quality has major economic impact. Cotton is a model for plant genome evolution, polyploidization and cellulose biosynthesis.Gene expression analysis using Agilent’s highly sensitive Cotton Microarray is a critical tool functional genomic research and breeding. Oligonucleotide quality, probe design and protocol flexibility allow us to obtain wide dynamic range, and unmatched sensitivity and reproducibility.