Better Separation Performance
Tetrandrine Extracted from Natural Products, Formulation
| Column A: Brand X Flash Irregular C18 (40-60 μm 100 Å, 12 g, 3 columns in tandem) |
| Column B: Claricep™ Spherical C18 (20-35 μm 100 Å, 12 g, 3 columns in tandem) |
| Column C: Claricep Spherical C18 (20 μm 100 Å, 12 g, 3 columns in tandem) |
| Mobile Phase: A: Water B: Methanol |
| Gradient: Time (min) 0 100 |
% B 85 85 |
| Flow Rate: 83 mL/min |
| Detector: UV @ 254/282 nm |
| Sample Tetrandrine |
Ink Sample Interference / Irregular vs. Spherical
Take 50 μL of two kinds of raw ink samples separately, filter through with a 0.22 μm Nylon Filter and then load onto individual flash columns.
| Column A: Claricep™ Irregular C18 (40-60 μm 60 Å, 20 g, 2 columns in tandem) |
| Column B: Claricep™ Spherical C18 (40-60 μm 100 Å, 20 g, 2 columns in tandem) |
| Mobile Phase: A: Water B: Methanol |
| Gradient: Time (min) 0 60 |
% B 10 100 |
| Flow Rate: 26 mL/min |
| Detector: UV@254 nm (red), @ 220 nm (blue) |
| Instrument: CHEETAH™ MP 200 |
Polypeptide Isolation
HPLC Analysis
| Column: LC C18, 5 μm, 100 Å, 4.6 × 150 mm |
| Mobile Phase: A: Water + 0.01 % TFA B: Acetonitrile (A/B, 73:27) |
| Flow Rate: 1 mL/min |
| Detector: UV @ 205 nm |
| Injection Volume: 1 μL |
Flash Purification
| Column: Claricep™ C18 (40-60 μ |
