Flash Spherical C18, Spherical; 20-35µm; 100A; 4g/branch, 5/pk
Бренд: Phenomenex
Better Separation Performance
Tetrandrine Extracted from Natural Products, Formulation
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Column A:
Brand X Flash Irregular C18 (40-60 μm 100 Å, 12 g, 3 columns in tandem)
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Column B:
Claricep™ Spherical C18 (20-35 μm 100 Å, 12 g, 3 columns in tandem)
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Column C:
Claricep Spherical C18 (20 μm 100 Å, 12 g, 3 columns in tandem)
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Mobile Phase:
A: Water B: Methanol
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Gradient:
Time (min)
0 100
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% B
85 85
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Flow Rate:
83 mL/min
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Detector:
UV @ 254/282 nm
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Sample
Tetrandrine
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Ink Sample Interference / Irregular vs. Spherical
Take 50 μL of two kinds of raw ink samples separately, filter through with a 0.22 μm Nylon Filter and then load onto individual flash columns.
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Column A:
Claricep™ Irregular C18 (40-60 μm 60 Å, 20 g, 2 columns in tandem)
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Column B:
Claricep™ Spherical C18 (40-60 μm 100 Å, 20 g, 2 columns in tandem)
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Mobile Phase:
A: Water B: Methanol
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Gradient:
Time (min)
0 60
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% B
10 100
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Flow Rate:
26 mL/min
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Detector:
UV@254 nm (red), @ 220 nm (blue)
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Instrument:
CHEETAH™ MP 200
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Polypeptide Isolation
HPLC Analysis
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Column:
LC C18, 5 μm, 100 Å, 4.6 × 150 mm
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Mobile Phase:
A: Water + 0.01 % TFA B: Acetonitrile (A/B, 73:27)
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Flow Rate:
1 mL/min
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Detector:
UV @ 205 nm
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Injection Volume:
1 μL
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Flash Purification
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Column:
Claricep™ C18 (40-60 μ
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