Name: GK80040 - AdvanceBio Sialidase A, 1 U
Brand: Agilent Technologies
SKU: GK80040
Availability: InStock

AdvanceBio Sialidase A, 1 U

Specifications

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Concentration	5 U/mL
Enzyme Formulation	20 mM Tris HCl pH 7.5, containing 25 mM NaCl.
Enzyme Source	Recombinant gene from Arthrobacter ureafaciens, expressed in E. coli. Sialidases are also known as neuraminidases.
Enzyme Specific Activity	40 units/mg
Enzyme Specificity	The enzyme releases a(2,3)-, a(2,6)-, a(2,8)-, and a(2,9)-linked N-acetylneuraminic acid from complex carbohydrates. The initial rate of hydrolysis of a(2,6) linkages is reported to be approximately twice that of a(2,3)-linked sialic acid however, in practice, this kinetic selectivity is of little consequence during extended incubations. Effective digestion of glycolipid substrates is facilitated by addition of a detergent, such as sodium taurodeoxycholate to the incubation. Sialidase A is capable of releasing N-glycolylneuraminic acid (Neu5Gc, NGNA) in addition to N-acetylneuraminic acid (Neu5Ac, NANA) [1], although similarly to other sialidases [2] the activity is lower toward Neu5Gc than Neu5Ac. The enzyme can also be used to remove sialic acid from gangliosides [3], glycosphingolipids (ceramide and oligosaccharide) with sialic acid. May be used for exoglycosidase sequencing with Sialidase S (GK80021), which is specific for a(2,3)-linked sialic acids. Sialidases are also known as neuraminidase.
Enzyme Unit Definition	One unit is defined as the amount of enzyme required to catalyze the release of 1 µmole of p-nitrophenol from p-nitrophenyl-α-D-N-acetylneuraminic acid per minute at 37° C, pH 5.5.
Molecular Weight	~88 kD
Unit	1 U
Volume	200 µL
pH Optimum	6

GK80040 — AdvanceBio Sialidase A, 1 U

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