GKE-5002 — AdvanceBio Endoglycosidase H

Name: GKE-5002 - AdvanceBio Endoglycosidase H
Brand: Agilent Technologies
SKU: GKE-5002
Availability: InStock

Бренд: Agilent Technologies

AdvanceBio Endoglycosidase H

Specifications

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Application	Analysis of biosynthesis of glycoproteins
Concentration	≥0.15 U/mL
Enzyme Applications	Selective release of high mannose N-linked oligosaccharides from glycopeptides/ glycoproteins. The enzyme has found extensive use in the characterization of glycoproteins, dolichol-linked glycans and the biosynthetic pathway for N-glycosylation.
Enzyme Formulation	50 mM sodium phosphate pH 7.5, 25 mM EDTA, and 0.05% sodium azide
Enzyme Source	Recombinant gene from Streptomyces plicatus, expressed in E. coli. Enzyme is also known as Endoglycosidase H, mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase.
Enzyme Unit Definition	One unit is defined as the amount of enzyme required to catalyze the release of N-linked oligosaccharides from 60 µmoles of denatured ribonuclease B in 1 hour at 37°C, pH 5.5.
Unit	30 mU
Volume	200 µL
pH Optimum	5.5