Name: GKE-5006B - AdvanceBio N-Glycanase (PNGase F), ≥2.5 U/mL
Brand: Agilent Technologies
SKU: GKE-5006B
Availability: InStock

AdvanceBio N-Glycanase (PNGase F), ≥2.5 U/mL

Part Number	GKE-5006B
Product Description	AdvanceBio N-Glycanase (PNGase F), ≥2.5 U/mL
Molecular Weight	~35,000 daltons
Volume	80 µL
Concentration	≥2.5 U/mL

Specifications

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Concentration	≥2.5 U/mL
Enzyme Applications	<!— "multilineKeyVal pipe value":Preparation of deglycosylated proteins for molecular weight estimation or crystallography studies Release of intact N-linked glycans from glycopeptides and glycoproteins Structure and function studies of N-glycosylated glycoproteins —> Preparation of deglycosylated proteins for molecular weight estimation or crystallography studies Release of intact N-linked glycans from glycopeptides and glycoproteins Structure and function studies of N-glycosylated glycoproteins
Enzyme Formulation	20 mM Tris-HCl, 50 mM NaCl, 1 mM EDTA (pH 7.5)
Enzyme Source	Recombinant gene from Elizabethkingia meningoseptica, expressed in E. coli. The source organism was previously known as Chryseobacterium [Flavobacterium] meningosepticum. The enzyme is also known as PNGase F, peptide-N-glycosidase F, peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase.
Enzyme Specific Activity	≥10 units/mg
Enzyme Specificity	Cleaves all N-linked complex, hybrid or high mannose oligosaccharides, unless α(1-3) core fucosylated, as in plant glycans and some insect glycans. Asparagine must be peptide-bonded at both termini. Phosphate, sulfate and sialic acid groups attached to the oligosaccharide do not affect cleavage. This product is Endo F free.
Enzyme Unit Definition	One unit is defined as the amount of enzyme required to catalyze the release of N-linked oligosaccharides from 1 μmole of denatured ribonuclease B per minute at 37°C, pH 7.5.
Molecular Weight	~35,000 daltons
Unit	200 mU
Volume	80 µL
pH Optimum	8.6
pH Range	7.5-9.5

GKE-5006B — AdvanceBio N-Glycanase (PNGase F), ≥2.5 U/mL

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