Name: GKE-5006D - AdvanceBio N-Glycanase (PNGase F), ≥2.5 U/mL
Brand: Agilent Technologies
SKU: GKE-5006D
Availability: InStock

AdvanceBio N-Glycanase (PNGase F), ≥2.5 U/mL

Part Number	GKE-5006D
Product Description	AdvanceBio N-Glycanase (PNGase F), ≥2.5 U/mL
Molecular Weight	~35,000 daltons
Volume	400 µL
Concentration	2.5 U/mL

Specifications

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Concentration	2.5 U/mL
Enzyme Applications	<!— "multilineKeyVal pipe value":Preparation of deglycosylated proteins for molecular weight estimation or crystallography studies Release of intact N-linked glycans from glycopeptides and glycoproteins Structure-function studies of N-glycosylated glycoproteins —> Preparation of deglycosylated proteins for molecular weight estimation or crystallography studies Release of intact N-linked glycans from glycopeptides and glycoproteins Structure-function studies of N-glycosylated glycoproteins
Enzyme Formulation	20 mM Tris-HCl, 50 mM NaCl, 1 mM EDTA (pH 7.5)
Enzyme Source	Recombinant gene from Elizabethkingia meningoseptica, expressed in E. coli. The source organism was previously known as Chryseobacterium [Flavobacterium] meningosepticum. Enzyme is also known as peptide-N-glycosidase F, peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase.
Enzyme Specific Activity	≥10 units/mg
Enzyme Specificity	Cleaves all N-linked complex, hybrid or high mannose oligosaccharides, unless a(1-3) core fucosylated, as in plant glycans and some insect glycans. Asparagine must be peptide bonded at both termini. Phosphate, sulfate and sialic acid groups attached to the oligosaccharide do not affect cleavage. Endo F free.
Enzyme Unit Definition	One unit is defined as the amount of enzyme required to catalyze the release of N-linked oligosaccharides from 1 µmole of denatured ribonuclease B per minute at 37°C, pH 7.5.
Molecular Weight	~35,000 daltons
Unit	1 U
Volume	400 µL
pH Optimum	8.6
pH Range	7.5-9.5

GKE-5006D — AdvanceBio N-Glycanase (PNGase F), ≥2.5 U/mL

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