Name: GKE-5011A - AdvanceBio PNGase A (almond), 2 mU
Brand: Agilent Technologies
SKU: GKE-5011A
Availability: InStock

AdvanceBio PNGase A (almond), 2 mU

Specifications

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Enzyme Formulation	Lyophilized powder containing citrate-phosphate buffer, pH 5.0.
Enzyme Source	Almond. Enzyme also knows as Peptide N-Glycosidase A, Glycopeptidase from almonds, Almond glycoamidase, Peptide-N(4)-(N-acetyl-β glucosaminyl) asparagine amidase.
Enzyme Specific Activity	> 5 units/mg
Enzyme Specificity	PNGase A from almonds cleaves N-glycan chains linked to Asparagine from glycopeptides. The enzyme hydrolyzes an N(4)-(acetyl-ß D-glucosaminyl) asparagine residue in which the glucosamine residue may be further glycosylated, to yield a (substituted) N-acetyl-ß D-glucosaminylamine and a peptide containing an aspartate residue. The enzyme cleaves N-glycans with or without a(1,3)-linked core fucose residues present in insect and plant glycoproteins. This differs from PNGase F, which cannot remove N-glycans with a(1,3)-linked core fucose. PNGase A requires glycopeptides as substrates. Glycopeptides can be obtained by digesting the glycoprotein with trypsin [1,2] or pepsin. Does not act on (GlcNAc)-Asn, because it requires the presence of more than two amino acid residues in the substrate.
Enzyme Unit Definition	One unit is defined as the amount of enzyme required to hydrolyze 1 µmol ovalbumin glycopeptide per min at pH 5.0 and 37°C.
Molecular Weight	54.2 and 21.2 kDa
Unit	2 mU
pH Optimum	5
pH Range	4-6

GKE-5011A — AdvanceBio PNGase A (almond), 2 mU