Name: GKE-5016A - AdvanceBio N-Glycanase (PNGase F, EDTA free), ≥2.5 U/mL
Brand: Agilent Technologies
SKU: GKE-5016A
Availability: InStock

AdvanceBio N-Glycanase (PNGase F, EDTA free), ≥2.5 U/mL

Specifications

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Concentration	2.5 U/mL
Enzyme Formulation	20 mM Tris-HCl, 50 mM NaCl, (pH 7.5)
Enzyme Source	Recombinant gene from Elizabethkingia meningoseptica, expressed in E. coli. The source organism was previously known as Chryseobacterium [Flavobacterium] meningosepticum. Enzyme is also known as Endoglycosidase F2, endo-beta-N-acetylglucosaminidase. Enzyme also known as PNGase F, peptide-N-glycosidase F, peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase.
Enzyme Specific Activity	≥10 units/mg
Enzyme Specificity	Cleaves all N-linked complex, hybrid or high mannose oligosaccharides, unless a(1-3) core fucosylated, as in plant glycans. Asparagine must be peptide bonded at both termini. Phosphate, sulfate and sialic acid groups attached to the oligosaccharide do not affect cleavage. Endo F free. Highly concentrated, particularly useful for deglycosylation under native conditions.
Enzyme Unit Definition	One unit is defined as the amount of enzyme required to catalyze the release of N-linked oligosaccharides from 1 µmole of denatured ribonuclease B per minute at 37°C, pH 7.5.
Molecular Weight	~35,000 Daltons
Unit	100 mU
Volume	40 µL
pH Optimum	8.6
pH Range	7.5-9.5

GKE-5016A — AdvanceBio N-Glycanase (PNGase F, EDTA free), ≥2.5 U/mL

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