Name: GKE-5020D-10 - AdvanceBio N-Glycanase-ultra (PNGase F, EDTA free), ≥10 U/mL
Brand: Agilent Technologies
SKU: GKE-5020D-10
Availability: InStock

AdvanceBio N-Glycanase-ultra (PNGase F, EDTA free), ≥10 U/mL

Specifications

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Concentration	≥10 U/mL
Enzyme Applications	To obtain efficient deglycosylation of glycoprotein substrates under non-denaturing conditions, it is necessary to use a higher starting concentration of enzyme. N-Glycanase PLUS and ULTRA (EDTA-Free) are supplied at = 10 U/ml, and are recommended for all applications requiring deglycosylation of glycoproteins in the absence of denaturants. The high activity also allows smaller reaction volumes and shorter reaction times to be explored.
Enzyme Formulation	20 mM Tris HCl pH 7.5, 50 mM NaCl
Enzyme Source	Recombinant gene from Elizabethkingia meningoseptica, expressed in E. coli. The source organism was previously known as Chryseobacterium [Flavobacterium] meningosepticum. Enzyme is also known as Endoglycosidase F2, endo-beta-N-acetylglucosaminidase. Enzyme also known as PNGase F, peptide-N-glycosidase F, peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase.
Enzyme Specific Activity	≥10 units/mg
Enzyme Specificity	Cleaves all N-linked complex, hybrid or high mannose oligosaccharides, unless a(1-3) core fucosylated, as in plant glycans. Asparagine must be peptide bonded at both termini. Phosphate, sulfate, and sialic acid groups attached to the oligosaccharide do not affect cleavage. Endo F free. Highly concentrated, particularly useful for deglycosylation under native conditions.
Enzyme Unit Definition	One unit is defined as the amount of enzyme required to catalyze the release of N-linked oligosaccharides from 1 µmole of denatured ribonuclease B per minute at 37°C, pH 7.5.
Molecular Weight	~35,000 Daltons
Unit	10 U
Volume	100 µL
pH Optimum	8.6
pH Range	7.5-9.5

GKE-5020D-10 — AdvanceBio N-Glycanase-ultra (PNGase F, EDTA free), ≥10 U/mL

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