GKX-5003 — β-N-Acetylhexosaminidase (jack bean)

Бренд: Agilent Technologies

β-N-Acetylhexosaminidase (jack bean)

Specifications

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Concentration
  • 50 U/mL
Enzyme Applications
  • Useful for the structural analysis of isolated glycans and glycoconjugates. pH optimum/range: For glucosaminide 5.0 — 6.0, For galactosaminide 3.5 — 4.0
Enzyme Formulation
  • 20mM sodium citrate phosphate (pH 6.0)
Enzyme Source
  • Jack bean.
Enzyme Specificity
  • The enzyme exhibits a broad specificity, cleaving nonreducing terminal ß(1-2,3,4,6)-linked N-acetylglucosamine (GlcNAc) and N-acetylgalactosamine (GalNAc) residues. This enzyme is useful in the study of isolated glycans, glycolipids and glycoproteins, especially in combination with ß N-acetylhexosaminidase from S. pneumoniae (GK80050). We also offer this enzyme at a higher concentration — see GKX-5023. Higher enzyme concentrations may be necessary to remove bisecting ß(1-4)-linked GlcNAc residues in complex N-glycans.
Enzyme Unit Definition
  • One unit is defined as the amount of enzyme required to hydrolyze 1 µmole of pNP N-acetyl-ß-D-glucosaminide per minute at pH 5.0 and 37°C.
Molecular Weight
  • 100 — 110 kDa
Unit
  • 5 U
Volume
  • 100 µL
Артикул: GKX-5003 Категория: Бренд: