Name: GKX-5013 - β(1-3,4)-Galactosidase (bovine testis)
Brand: Agilent Technologies
SKU: GKX-5013
Availability: InStock

β(1-3,4)-Galactosidase (bovine testis)

Specifications

<!—

—>


Concentration	5 U/mL
Enzyme Applications	The enzyme has applications in the analysis of a wide variety of glycoconjugates. It is particularly useful for ensuring the complete removal of ß(1-3) and ß(1-4)-linked non-reducing terminal galactose residues of oligosaccharides. Gal ß(1-6) GlcNAc is hydrolyzed more slowly, however this linkage is not normally encountered in native complex glycans. This activity towards ß(1-3) and ß(1-4)-linked galactose contrasts with that of our other ß-galactosidases (e.g. from S. pneumoniae, GKX-5014, or Jack bean, GKX-5012) which exhibit a preference for Gal ß(1-4), and cleave the Gal ß(1-3) linkage relatively slowly, if at all. Used in conjunction, these enzymes provide a powerful means to determine linkage positions of non-reducing ß galactose residues.
Enzyme Formulation	20 mM sodium citrate phosphate, 150 mM NaCl (pH 4.0)
Enzyme Source	Bovine testis.
Enzyme Unit Definition	One unit is defined as the amount of enzyme required to hydrolyze 1 µmole of pNP-ß-D-galactopyranoside per minute at pH 4.0 and 37°C.
Molecular Weight	~68 kD
Unit	0.5 U
Volume	100 µL
pH Optimum	4

GKX-5013 — β(1-3,4)-Galactosidase (bovine testis)