Re-imagine a streamlined, fast, and highly sensitive way to analyze released N-glycans. With just 3 easy steps in as little as 30 minutes, the GlycoWorks RapiFluor-MS N-Glycan Kit reduces complicated, time-consuming sample preparation. RapiFluor-MS’s (RFMS) unique chemical attributes provide increased fluorescence quantification and supreme mass spectral response. Now you can use a single label that provides valuable information from characterization to routine monitoring. Glycan analysis just got easier and more sensitive.
This starter kit includes the GlycoWorks Deglycosylation Module, GlycoWorks Labeling Module, GlycoWorks Clean-up Module, GlycoWorks Sample Collection Module, as well as the ACQUITY UPLC Glycan BEH Amide, 1.7 ìm, 2.1 x 150 Column and Mobile-Phase Concentrate: Ammonium Formate.
It contains materials to process 96 samples in an 8×12 format.
The kit contains:
GlycoWorks Deglycosylation Module (4 Boxes):
(1) 0.035 ml/vial Rapid PNGaseF Enzyme
(1) 0.25 ml/vial Glycoworks Rapid Buffer
(1) 3 vials of 3 mg/vial RapiGest™ SF Surfactant
(1) Intact mAb Mass Check Standard *Shipped in a separate box only one per kit
GlycoWorks RapiFluor-MS Labeling Module (4 Boxes):
(3) 9 mg/vial RapiFluor-MS Reagent
(3) 1 mL/vial Reagent Solvent (Anhydrous DMF)
GlycoWorks RFMS Clean-up Module:
(4) 0.5 ml/vial Elution Buffer (200mM Ammonium Acetate 95/5 Water/ACN pH 7)
(4) 3 ml/vial Sample Diluent (DMF/ACN Mixture)
(1) GlycoWorks HILIC µElution Plate
GlycoWorks Sample Collection Module:
(15) 1 mL tubes for deglycosylation and labeling reaction
(15) 600 µL tube inserts for SPE eluate collection
(15) Cap strips with slits for direct injection
(1) 1 mL round collection tray
(1) Waste tray
In Addition To:
(1) 5050 mM Ammonium Formate Solution—Glycan Analysis (186007081)
(1) ACQUITY UPLC Glycan BEH Amide Column, 130A, 1.7ìm, 2.1 x 150 mm (186004742)
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Number of Analysis
96
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Type
Sample Preparation Workflow
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Packages per kit
7
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Separation Mode
HILIC
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Detection Method
MS, MS/MS, UV, HRMS
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Storage Condition
Refer to the storage recommendations of each sub-package
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System Type
LC-MS
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Shelf Life
Refer to the storage recommendations of each sub-package
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UNSPSC
41116104
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Application
Glycan
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Brand
GlycoWorks
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Product Type
Application Kits
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Units per Package
1 pk