<!— "inside defaultSpecification":{Application|MultiLine=Flexible, high-throughput format: process 1 to 24 samples|Reconstituted InstantPC dye may be stored frozen (-20°C) for up to 3 months.|Small molecule size for improved chromatographic peak resolution|, Count=24, Deglycosylation Efficiency=Complete for most proteins. Protein load range: *More than 40 μg glycoprotein may be used, depending on the extent of glycosylation. %, Equipment & Reagents Provided by User|MultiLine=96-well Thermocycler or two independent heat blocks, set for 90 °C and 50 °C|Note: Two GlykoPrep heaters, WS0271, can be fitted with VWR 13259-260 modular heating blocks|THERM-1000, 230V)|Thermocycler (Corning THERM-1001, 110V|, Kit Configuration=Sample Prep Module Set, Label=InstantPC, Precision (well-to-well, day-to-day)= 95 %, Protein Load Range=1-40 µg, Recovery=> 95 %, Stability=Samples may be stored at -20°C for up to 6 months in Gly-X InstantPC eluent, or 4°C for up to 5 days in either Gly-X InstantPC eluent or after dilution with ACN/DMF, Technique=LC & LC/MS}—>
| Application |
|
<!— "inside defaultSpecification":{Application|MultiLine=Flexible, high-throughput format: process 1 to 24 samples|Reconstituted InstantPC dye may be stored frozen (-20°C) for up to 3 months.|Small molecule size for improved chromatographic peak resolution|, Count=24, Deglycosylation Efficiency=Complete for most proteins. Protein load range: *More than 40 μg glycoprotein may be used, depending on the extent of glycosylation. %, Equipment & Reagents Provided by User|MultiLine=96-well Thermocycler or two independent heat blocks, set for 90 °C and 50 °C|Note: Two GlykoPrep heaters, WS0271, can be fitted with VWR 13259-260 modular heating blocks|THERM-1000, 230V)|Thermocycler (Corning THERM-1001, 110V|, Kit Configuration=Sample Prep Module Set, Label=InstantPC, Precision (well-to-well, day-to-day)= 95 %, Protein Load Range=1-40 µg, Recovery=> 95 %, Stability=Samples may be stored at -20°C for up to 6 months in Gly-X InstantPC eluent, or 4°C for up to 5 days in either Gly-X InstantPC eluent or after dilution with ACN/DMF, Technique=LC & LC/MS}—>
| Count |
|
<!— "inside defaultSpecification":{Application|MultiLine=Flexible, high-throughput format: process 1 to 24 samples|Reconstituted InstantPC dye may be stored frozen (-20°C) for up to 3 months.|Small molecule size for improved chromatographic peak resolution|, Count=24, Deglycosylation Efficiency=Complete for most proteins. Protein load range: *More than 40 μg glycoprotein may be used, depending on the extent of glycosylation. %, Equipment & Reagents Provided by User|MultiLine=96-well Thermocycler or two independent heat blocks, set for 90 °C and 50 °C|Note: Two GlykoPrep heaters, WS0271, can be fitted with VWR 13259-260 modular heating blocks|THERM-1000, 230V)|Thermocycler (Corning THERM-1001, 110V|, Kit Configuration=Sample Prep Module Set, Label=InstantPC, Precision (well-to-well, day-to-day)= 95 %, Protein Load Range=1-40 µg, Recovery=> 95 %, Stability=Samples may be stored at -20°C for up to 6 months in Gly-X InstantPC eluent, or 4°C for up to 5 days in either Gly-X InstantPC eluent or after dilution with ACN/DMF, Technique=LC & LC/MS}—>
| Deglycosylation Efficiency |
- Complete for most proteins. Protein load range: *More than 40 μg glycoprotein may be used, depending on the extent of glycosylation. %
|
<!— "inside defaultSpecification":{Application|MultiLine=Flexible, high-throughput format: process 1 to 24 samples|Reconstituted InstantPC dye may be stored frozen (-20°C) for up to 3 months.|Small molecule size for improved chromatographic peak resolution|, Count=24, Deglycosylation Efficiency=Complete for most proteins. Protein load range: *More than 40 μg glycoprotein may be used, depending on the extent of glycosylation. %, Equipment & Reagents Provided by User|MultiLine=96-well Thermocycler or two independent heat blocks, set for 90 °C and 50 °C|Note: Two GlykoPrep heaters, WS0271, can be fitted with VWR 13259-260 modular heating blocks|THERM-1000, 230V)|Thermocycler (Corning THERM-1001, 110V|, Kit Configuration=Sample Prep Module Set, Label=InstantPC, Precision (well-to-well, day-to-day)= 95 %, Protein Load Range=1-40 µg, Recovery=> 95 %, Stability=Samples may be stored at -20°C for up to 6 months in Gly-X InstantPC eluent, or 4°C for up to 5 days in either Gly-X InstantPC eluent or after dilution with ACN/DMF, Technique=LC & LC/MS}—>
| Equipment & Reagents Provided by User |
|
<!— "inside defaultSpecification":{Application|MultiLine=Flexible, high-throughput format: process 1 to 24 samples|Reconstituted InstantPC dye may be stored frozen (-20°C) for up to 3 months.|Small molecule size for improved chromatographic peak resolution|, Count=24, Deglycosylation Efficiency=Complete for most proteins. Protein load range: *More than 40 μg glycoprotein may be used, depending on the extent of glycosylation. %, Equipment & Reagents Provided by User|MultiLine=96-well Thermocycler or two independent heat blocks, set for 90 °C and 50 °C|Note: Two GlykoPrep heaters, WS0271, can be fitted with VWR 13259-260 modular heating blocks|THERM-1000, 230V)|Thermocycler (Corning THERM-1001, 110V|, Kit Configuration=Sample Prep Module Set, Label=InstantPC, Precision (well-to-well, day-to-day)= 95 %, Protein Load Range=1-40 µg, Recovery=> 95 %, Stability=Samples may be stored at -20°C for up to 6 months in Gly-X InstantPC eluent, or 4°C for up to 5 days in either Gly-X InstantPC eluent or after dilution with ACN/DMF, Technique=LC & LC/MS}—>
| Kit Configuration |
|
<!— "inside defaultSpecification":{Application|MultiLine=Flexible, high-throughput format: process 1 to 24 samples|Reconstituted InstantPC dye may be stored frozen (-20°C) for up to 3 months.|Small molecule size for improved chromatographic peak resolution|, Count=24, Deglycosylation Efficiency=Complete for most proteins. Protein load range: *More than 40 μg glycoprotein may be used, depending on the extent of glycosylation. %, Equipment & Reagents Provided by User|MultiLine=96-well Thermocycler or two independent heat blocks, set for 90 °C and 50 °C|Note: Two GlykoPrep heaters, WS0271, can be fitted with VWR 13259-260 modular heating blocks|THERM-1000, 230V)|Thermocycler (Corning THERM-1001, 110V|, Kit Configuration=Sample Prep Module Set, Label=InstantPC, Precision (well-to-well, day-to-day)= 95 %, Protein Load Range=1-40 µg, Recovery=> 95 %, Stability=Samples may be stored at -20°C for up to 6 months in Gly-X InstantPC eluent, or 4°C for up to 5 days in either Gly-X InstantPC eluent or after dilution with ACN/DMF, Technique=LC & LC/MS}—>
| Label |
|
<!— "inside defaultSpecification":{Application|MultiLine=Flexible, high-throughput format: process 1 to 24 samples|Reconstituted InstantPC dye may be stored frozen (-20°C) for up to 3 months.|Small molecule size for improved chromatographic peak resolution|, Count=24, Deglycosylation Efficiency=Complete for most proteins. Protein load range: *More than 40 μg glycoprotein may be used, depending on the extent of glycosylation. %, Equipment & Reagents Provided by User|MultiLine=96-well Thermocycler or two independent heat blocks, set for 90 °C and 50 °C|Note: Two GlykoPrep heaters, WS0271, can be fitted with VWR 13259-260 modular heating blocks|THERM-1000, 230V)|Thermocycler (Corning THERM-1001, 110V|, Kit Configuration=Sample Prep Module Set, Label=InstantPC, Precision (well-to-well, day-to-day)= 95 %, Protein Load Range=1-40 µg, Recovery=> 95 %, Stability=Samples may be stored at -20°C for up to 6 months in Gly-X InstantPC eluent, or 4°C for up to 5 days in either Gly-X InstantPC eluent or after dilution with ACN/DMF, Technique=LC & LC/MS}—>
| Precision (well-to-well, day-to-day) |
- <5% CV
<!— Precision (well-to-well, day-to-day)::
|
<!— "inside defaultSpecification":{Application|MultiLine=Flexible, high-throughput format: process 1 to 24 samples|Reconstituted InstantPC dye may be stored frozen (-20°C) for up to 3 months.|Small molecule size for improved chromatographic peak resolution|, Count=24, Deglycosylation Efficiency=Complete for most proteins. Protein load range: *More than 40 μg glycoprotein may be used, depending on the extent of glycosylation. %, Equipment & Reagents Provided by User|MultiLine=96-well Thermocycler or two independent heat blocks, set for 90 °C and 50 °C|Note: Two GlykoPrep heaters, WS0271, can be fitted with VWR 13259-260 modular heating blocks|THERM-1000, 230V)|Thermocycler (Corning THERM-1001, 110V|, Kit Configuration=Sample Prep Module Set, Label=InstantPC, Precision (well-to-well, day-to-day)= 95 %, Protein Load Range=1-40 µg, Recovery=> 95 %, Stability=Samples may be stored at -20°C for up to 6 months in Gly-X InstantPC eluent, or 4°C for up to 5 days in either Gly-X InstantPC eluent or after dilution with ACN/DMF, Technique=LC & LC/MS}—>
| Prep time (approx) |
- 42 min (16 samples), 99 min (96 samples)
|
<!— "inside defaultSpecification":{Application|MultiLine=Flexible, high-throughput format: process 1 to 24 samples|Reconstituted InstantPC dye may be stored frozen (-20°C) for up to 3 months.|Small molecule size for improved chromatographic peak resolution|, Count=24, Deglycosylation Efficiency=Complete for most proteins. Protein load range: *More than 40 μg glycoprotein may be used, depending on the extent of glycosylation. %, Equipment & Reagents Provided by User|MultiLine=96-well Thermocycler or two independent heat blocks, set for 90 °C and 50 °C|Note: Two GlykoPrep heaters, WS0271, can be fitted with VWR 13259-260 modular heating blocks|THERM-1000, 230V)|Thermocycler (Corning THERM-1001, 110V|, Kit Configuration=Sample Prep Module Set, Label=InstantPC, Precision (well-to-well, day-to-day)= 95 %, Protein Load Range=1-40 µg, Recovery=> 95 %, Stability=Samples may be stored at -20°C for up to 6 months in Gly-X InstantPC eluent, or 4°C for up to 5 days in either Gly-X InstantPC eluent or after dilution with ACN/DMF, Technique=LC & LC/MS}—>
| Preservation of Sialylated Species |
|
<!— "inside defaultSpecification":{Application|MultiLine=Flexible, high-throughput format: process 1 to 24 samples|Reconstituted InstantPC dye may be stored frozen (-20°C) for up to 3 months.|Small molecule size for improved chromatographic peak resolution|, Count=24, Deglycosylation Efficiency=Complete for most proteins. Protein load range: *More than 40 μg glycoprotein may be used, depending on the extent of glycosylation. %, Equipment & Reagents Provided by User|MultiLine=96-well Thermocycler or two independent heat blocks, set for 90 °C and 50 °C|Note: Two GlykoPrep heaters, WS0271, can be fitted with VWR 13259-260 modular heating blocks|THERM-1000, 230V)|Thermocycler (Corning THERM-1001, 110V|, Kit Configuration=Sample Prep Module Set, Label=InstantPC, Precision (well-to-well, day-to-day)= 95 %, Protein Load Range=1-40 µg, Recovery=> 95 %, Stability=Samples may be stored at -20°C for up to 6 months in Gly-X InstantPC eluent, or 4°C for up to 5 days in either Gly-X InstantPC eluent or after dilution with ACN/DMF, Technique=LC & LC/MS}—>
| Protein Load Range |
|
<!— "inside defaultSpecification":{Application|MultiLine=Flexible, high-throughput format: process 1 to 24 samples|Reconstituted InstantPC dye may be stored frozen (-20°C) for up to 3 months.|Small molecule size for improved chromatographic peak resolution|, Count=24, Deglycosylation Efficiency=Complete for most proteins. Protein load range: *More than 40 μg glycoprotein may be used, depending on the extent of glycosylation. %, Equipment & Reagents Provided by User|MultiLine=96-well Thermocycler or two independent heat blocks, set for 90 °C and 50 °C|Note: Two GlykoPrep heaters, WS0271, can be fitted with VWR 13259-260 modular heating blocks|THERM-1000, 230V)|Thermocycler (Corning THERM-1001, 110V|, Kit Configuration=Sample Prep Module Set, Label=InstantPC, Precision (well-to-well, day-to-day)= 95 %, Protein Load Range=1-40 µg, Recovery=> 95 %, Stability=Samples may be stored at -20°C for up to 6 months in Gly-X InstantPC eluent, or 4°C for up to 5 days in either Gly-X InstantPC eluent or after dilution with ACN/DMF, Technique=LC & LC/MS}—>
| Recovery |
|
<!— "inside defaultSpecification":{Application|MultiLine=Flexible, high-throughput format: process 1 to 24 samples|Reconstituted InstantPC dye may be stored frozen (-20°C) for up to 3 months.|Small molecule size for improved chromatographic peak resolution|, Count=24, Deglycosylation Efficiency=Complete for most proteins. Protein load range: *More than 40 μg glycoprotein may be used, depending on the extent of glycosylation. %, Equipment & Reagents Provided by User|MultiLine=96-well Thermocycler or two independent heat blocks, set for 90 °C and 50 °C|Note: Two GlykoPrep heaters, WS0271, can be fitted with VWR 13259-260 modular heating blocks|THERM-1000, 230V)|Thermocycler (Corning THERM-1001, 110V|, Kit Configuration=Sample Prep Module Set, Label=InstantPC, Precision (well-to-well, day-to-day)= 95 %, Protein Load Range=1-40 µg, Recovery=> 95 %, Stability=Samples may be stored at -20°C for up to 6 months in Gly-X InstantPC eluent, or 4°C for up to 5 days in either Gly-X InstantPC eluent or after dilution with ACN/DMF, Technique=LC & LC/MS}—>
| Stability |
- Samples may be stored at -20°C for up to 6 months in Gly-X InstantPC eluent, or 4°C for up to 5 days in either Gly-X InstantPC eluent or after dilution with ACN/DMF
|
<!— "inside defaultSpecification":{Application|MultiLine=Flexible, high-throughput format: process 1 to 24 samples|Reconstituted InstantPC dye may be stored frozen (-20°C) for up to 3 months.|Small molecule size for improved chromatographic peak resolution|, Count=24, Deglycosylation Efficiency=Complete for most proteins. Protein load range: *More than 40 μg glycoprotein may be used, depending on the extent of glycosylation. %, Equipment & Reagents Provided by User|MultiLine=96-well Thermocycler or two independent heat blocks, set for 90 °C and 50 °C|Note: Two GlykoPrep heaters, WS0271, can be fitted with VWR 13259-260 modular heating blocks|THERM-1000, 230V)|Thermocycler (Corning THERM-1001, 110V|, Kit Configuration=Sample Prep Module Set, Label=InstantPC, Precision (well-to-well, day-to-day)= 95 %, Protein Load Range=1-40 µg, Recovery=> 95 %, Stability=Samples may be stored at -20°C for up to 6 months in Gly-X InstantPC eluent, or 4°C for up to 5 days in either Gly-X InstantPC eluent or after dilution with ACN/DMF, Technique=LC & LC/MS}—>
| Technique |
|
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—>
