<!— "inside defaultSpecification":{Application|MultiLine=Flexible, high-throughput format: process 1 to 96 samples|Optimized cleanup removes excess free dye, protein, and other interfering compounds|Reconstituted InstantPC dye may be stored frozen (-20°C) for up to 3 months|Small molecule size for improved chromatographic peak resolution|, Count=96, Equipment & Reagents Provided by User|MultiLine=96-well Thermocycler or two independent heat blocks, set for 90°C and 50°C. (e.g. Corning THERM-1001, 110V. THERM-1000, 230V). Note: Two GlykoPrep heaters, WS0271, can be fitted with VWR 13259-260 modular heating blocks|Acetonitrile (ACN), MS-grade (Fisher A955-4 recommended)|Formic acid, MS-grade (Fisher A117-50 recommended)|Gly-X vacuum manifold spacer (Agilent GX100)|Optional — VWR 10 kDa centrifugal filters (82031-348)|Vacuum manifold (Millipore MSVMHTS00)|Vacuum pump (Millipore WP6211560, 110 V, WP6122050, 220V, Welch WOB-L Pump 2522). Note: If you have a vacuum manifold and pump other than the Millipore model suggested, please contact us for information. Alternative vacuum manifold may be used with a GX200 spacer in place of GX100 (see FAQs)|, Kit Configuration=Sample Prep Kit, Label=InstantPC, Precision (well-to-well, day-to-day)= 95 %, Protein Load Range=1-40 µg, Recovery=> 95 %, Stability=Samples may be stored at -20°C for up to 6 months in Gly-X InstantPC eluent, or 4°C for up to 5 days in either Gly-X InstantPC eluent or after dilution with ACN/DMF, Technique=LC & LC/MS}—>
Application |
|
<!— "inside defaultSpecification":{Application|MultiLine=Flexible, high-throughput format: process 1 to 96 samples|Optimized cleanup removes excess free dye, protein, and other interfering compounds|Reconstituted InstantPC dye may be stored frozen (-20°C) for up to 3 months|Small molecule size for improved chromatographic peak resolution|, Count=96, Equipment & Reagents Provided by User|MultiLine=96-well Thermocycler or two independent heat blocks, set for 90°C and 50°C. (e.g. Corning THERM-1001, 110V. THERM-1000, 230V). Note: Two GlykoPrep heaters, WS0271, can be fitted with VWR 13259-260 modular heating blocks|Acetonitrile (ACN), MS-grade (Fisher A955-4 recommended)|Formic acid, MS-grade (Fisher A117-50 recommended)|Gly-X vacuum manifold spacer (Agilent GX100)|Optional — VWR 10 kDa centrifugal filters (82031-348)|Vacuum manifold (Millipore MSVMHTS00)|Vacuum pump (Millipore WP6211560, 110 V, WP6122050, 220V, Welch WOB-L Pump 2522). Note: If you have a vacuum manifold and pump other than the Millipore model suggested, please contact us for information. Alternative vacuum manifold may be used with a GX200 spacer in place of GX100 (see FAQs)|, Kit Configuration=Sample Prep Kit, Label=InstantPC, Precision (well-to-well, day-to-day)= 95 %, Protein Load Range=1-40 µg, Recovery=> 95 %, Stability=Samples may be stored at -20°C for up to 6 months in Gly-X InstantPC eluent, or 4°C for up to 5 days in either Gly-X InstantPC eluent or after dilution with ACN/DMF, Technique=LC & LC/MS}—>
Count |
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<!— "inside defaultSpecification":{Application|MultiLine=Flexible, high-throughput format: process 1 to 96 samples|Optimized cleanup removes excess free dye, protein, and other interfering compounds|Reconstituted InstantPC dye may be stored frozen (-20°C) for up to 3 months|Small molecule size for improved chromatographic peak resolution|, Count=96, Equipment & Reagents Provided by User|MultiLine=96-well Thermocycler or two independent heat blocks, set for 90°C and 50°C. (e.g. Corning THERM-1001, 110V. THERM-1000, 230V). Note: Two GlykoPrep heaters, WS0271, can be fitted with VWR 13259-260 modular heating blocks|Acetonitrile (ACN), MS-grade (Fisher A955-4 recommended)|Formic acid, MS-grade (Fisher A117-50 recommended)|Gly-X vacuum manifold spacer (Agilent GX100)|Optional — VWR 10 kDa centrifugal filters (82031-348)|Vacuum manifold (Millipore MSVMHTS00)|Vacuum pump (Millipore WP6211560, 110 V, WP6122050, 220V, Welch WOB-L Pump 2522). Note: If you have a vacuum manifold and pump other than the Millipore model suggested, please contact us for information. Alternative vacuum manifold may be used with a GX200 spacer in place of GX100 (see FAQs)|, Kit Configuration=Sample Prep Kit, Label=InstantPC, Precision (well-to-well, day-to-day)= 95 %, Protein Load Range=1-40 µg, Recovery=> 95 %, Stability=Samples may be stored at -20°C for up to 6 months in Gly-X InstantPC eluent, or 4°C for up to 5 days in either Gly-X InstantPC eluent or after dilution with ACN/DMF, Technique=LC & LC/MS}—>
Equipment & Reagents Provided by User |
|
<!— "inside defaultSpecification":{Application|MultiLine=Flexible, high-throughput format: process 1 to 96 samples|Optimized cleanup removes excess free dye, protein, and other interfering compounds|Reconstituted InstantPC dye may be stored frozen (-20°C) for up to 3 months|Small molecule size for improved chromatographic peak resolution|, Count=96, Equipment & Reagents Provided by User|MultiLine=96-well Thermocycler or two independent heat blocks, set for 90°C and 50°C. (e.g. Corning THERM-1001, 110V. THERM-1000, 230V). Note: Two GlykoPrep heaters, WS0271, can be fitted with VWR 13259-260 modular heating blocks|Acetonitrile (ACN), MS-grade (Fisher A955-4 recommended)|Formic acid, MS-grade (Fisher A117-50 recommended)|Gly-X vacuum manifold spacer (Agilent GX100)|Optional — VWR 10 kDa centrifugal filters (82031-348)|Vacuum manifold (Millipore MSVMHTS00)|Vacuum pump (Millipore WP6211560, 110 V, WP6122050, 220V, Welch WOB-L Pump 2522). Note: If you have a vacuum manifold and pump other than the Millipore model suggested, please contact us for information. Alternative vacuum manifold may be used with a GX200 spacer in place of GX100 (see FAQs)|, Kit Configuration=Sample Prep Kit, Label=InstantPC, Precision (well-to-well, day-to-day)= 95 %, Protein Load Range=1-40 µg, Recovery=> 95 %, Stability=Samples may be stored at -20°C for up to 6 months in Gly-X InstantPC eluent, or 4°C for up to 5 days in either Gly-X InstantPC eluent or after dilution with ACN/DMF, Technique=LC & LC/MS}—>
Kit Configuration |
|
<!— "inside defaultSpecification":{Application|MultiLine=Flexible, high-throughput format: process 1 to 96 samples|Optimized cleanup removes excess free dye, protein, and other interfering compounds|Reconstituted InstantPC dye may be stored frozen (-20°C) for up to 3 months|Small molecule size for improved chromatographic peak resolution|, Count=96, Equipment & Reagents Provided by User|MultiLine=96-well Thermocycler or two independent heat blocks, set for 90°C and 50°C. (e.g. Corning THERM-1001, 110V. THERM-1000, 230V). Note: Two GlykoPrep heaters, WS0271, can be fitted with VWR 13259-260 modular heating blocks|Acetonitrile (ACN), MS-grade (Fisher A955-4 recommended)|Formic acid, MS-grade (Fisher A117-50 recommended)|Gly-X vacuum manifold spacer (Agilent GX100)|Optional — VWR 10 kDa centrifugal filters (82031-348)|Vacuum manifold (Millipore MSVMHTS00)|Vacuum pump (Millipore WP6211560, 110 V, WP6122050, 220V, Welch WOB-L Pump 2522). Note: If you have a vacuum manifold and pump other than the Millipore model suggested, please contact us for information. Alternative vacuum manifold may be used with a GX200 spacer in place of GX100 (see FAQs)|, Kit Configuration=Sample Prep Kit, Label=InstantPC, Precision (well-to-well, day-to-day)= 95 %, Protein Load Range=1-40 µg, Recovery=> 95 %, Stability=Samples may be stored at -20°C for up to 6 months in Gly-X InstantPC eluent, or 4°C for up to 5 days in either Gly-X InstantPC eluent or after dilution with ACN/DMF, Technique=LC & LC/MS}—>
Label |
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<!— "inside defaultSpecification":{Application|MultiLine=Flexible, high-throughput format: process 1 to 96 samples|Optimized cleanup removes excess free dye, protein, and other interfering compounds|Reconstituted InstantPC dye may be stored frozen (-20°C) for up to 3 months|Small molecule size for improved chromatographic peak resolution|, Count=96, Equipment & Reagents Provided by User|MultiLine=96-well Thermocycler or two independent heat blocks, set for 90°C and 50°C. (e.g. Corning THERM-1001, 110V. THERM-1000, 230V). Note: Two GlykoPrep heaters, WS0271, can be fitted with VWR 13259-260 modular heating blocks|Acetonitrile (ACN), MS-grade (Fisher A955-4 recommended)|Formic acid, MS-grade (Fisher A117-50 recommended)|Gly-X vacuum manifold spacer (Agilent GX100)|Optional — VWR 10 kDa centrifugal filters (82031-348)|Vacuum manifold (Millipore MSVMHTS00)|Vacuum pump (Millipore WP6211560, 110 V, WP6122050, 220V, Welch WOB-L Pump 2522). Note: If you have a vacuum manifold and pump other than the Millipore model suggested, please contact us for information. Alternative vacuum manifold may be used with a GX200 spacer in place of GX100 (see FAQs)|, Kit Configuration=Sample Prep Kit, Label=InstantPC, Precision (well-to-well, day-to-day)= 95 %, Protein Load Range=1-40 µg, Recovery=> 95 %, Stability=Samples may be stored at -20°C for up to 6 months in Gly-X InstantPC eluent, or 4°C for up to 5 days in either Gly-X InstantPC eluent or after dilution with ACN/DMF, Technique=LC & LC/MS}—>
Precision (well-to-well, day-to-day) |
- <5% CV
<!— Precision (well-to-well, day-to-day)::
|
<!— "inside defaultSpecification":{Application|MultiLine=Flexible, high-throughput format: process 1 to 96 samples|Optimized cleanup removes excess free dye, protein, and other interfering compounds|Reconstituted InstantPC dye may be stored frozen (-20°C) for up to 3 months|Small molecule size for improved chromatographic peak resolution|, Count=96, Equipment & Reagents Provided by User|MultiLine=96-well Thermocycler or two independent heat blocks, set for 90°C and 50°C. (e.g. Corning THERM-1001, 110V. THERM-1000, 230V). Note: Two GlykoPrep heaters, WS0271, can be fitted with VWR 13259-260 modular heating blocks|Acetonitrile (ACN), MS-grade (Fisher A955-4 recommended)|Formic acid, MS-grade (Fisher A117-50 recommended)|Gly-X vacuum manifold spacer (Agilent GX100)|Optional — VWR 10 kDa centrifugal filters (82031-348)|Vacuum manifold (Millipore MSVMHTS00)|Vacuum pump (Millipore WP6211560, 110 V, WP6122050, 220V, Welch WOB-L Pump 2522). Note: If you have a vacuum manifold and pump other than the Millipore model suggested, please contact us for information. Alternative vacuum manifold may be used with a GX200 spacer in place of GX100 (see FAQs)|, Kit Configuration=Sample Prep Kit, Label=InstantPC, Precision (well-to-well, day-to-day)= 95 %, Protein Load Range=1-40 µg, Recovery=> 95 %, Stability=Samples may be stored at -20°C for up to 6 months in Gly-X InstantPC eluent, or 4°C for up to 5 days in either Gly-X InstantPC eluent or after dilution with ACN/DMF, Technique=LC & LC/MS}—>
Prep time (approx) |
|
<!— "inside defaultSpecification":{Application|MultiLine=Flexible, high-throughput format: process 1 to 96 samples|Optimized cleanup removes excess free dye, protein, and other interfering compounds|Reconstituted InstantPC dye may be stored frozen (-20°C) for up to 3 months|Small molecule size for improved chromatographic peak resolution|, Count=96, Equipment & Reagents Provided by User|MultiLine=96-well Thermocycler or two independent heat blocks, set for 90°C and 50°C. (e.g. Corning THERM-1001, 110V. THERM-1000, 230V). Note: Two GlykoPrep heaters, WS0271, can be fitted with VWR 13259-260 modular heating blocks|Acetonitrile (ACN), MS-grade (Fisher A955-4 recommended)|Formic acid, MS-grade (Fisher A117-50 recommended)|Gly-X vacuum manifold spacer (Agilent GX100)|Optional — VWR 10 kDa centrifugal filters (82031-348)|Vacuum manifold (Millipore MSVMHTS00)|Vacuum pump (Millipore WP6211560, 110 V, WP6122050, 220V, Welch WOB-L Pump 2522). Note: If you have a vacuum manifold and pump other than the Millipore model suggested, please contact us for information. Alternative vacuum manifold may be used with a GX200 spacer in place of GX100 (see FAQs)|, Kit Configuration=Sample Prep Kit, Label=InstantPC, Precision (well-to-well, day-to-day)= 95 %, Protein Load Range=1-40 µg, Recovery=> 95 %, Stability=Samples may be stored at -20°C for up to 6 months in Gly-X InstantPC eluent, or 4°C for up to 5 days in either Gly-X InstantPC eluent or after dilution with ACN/DMF, Technique=LC & LC/MS}—>
Preservation of Sialylated Species |
|
<!— "inside defaultSpecification":{Application|MultiLine=Flexible, high-throughput format: process 1 to 96 samples|Optimized cleanup removes excess free dye, protein, and other interfering compounds|Reconstituted InstantPC dye may be stored frozen (-20°C) for up to 3 months|Small molecule size for improved chromatographic peak resolution|, Count=96, Equipment & Reagents Provided by User|MultiLine=96-well Thermocycler or two independent heat blocks, set for 90°C and 50°C. (e.g. Corning THERM-1001, 110V. THERM-1000, 230V). Note: Two GlykoPrep heaters, WS0271, can be fitted with VWR 13259-260 modular heating blocks|Acetonitrile (ACN), MS-grade (Fisher A955-4 recommended)|Formic acid, MS-grade (Fisher A117-50 recommended)|Gly-X vacuum manifold spacer (Agilent GX100)|Optional — VWR 10 kDa centrifugal filters (82031-348)|Vacuum manifold (Millipore MSVMHTS00)|Vacuum pump (Millipore WP6211560, 110 V, WP6122050, 220V, Welch WOB-L Pump 2522). Note: If you have a vacuum manifold and pump other than the Millipore model suggested, please contact us for information. Alternative vacuum manifold may be used with a GX200 spacer in place of GX100 (see FAQs)|, Kit Configuration=Sample Prep Kit, Label=InstantPC, Precision (well-to-well, day-to-day)= 95 %, Protein Load Range=1-40 µg, Recovery=> 95 %, Stability=Samples may be stored at -20°C for up to 6 months in Gly-X InstantPC eluent, or 4°C for up to 5 days in either Gly-X InstantPC eluent or after dilution with ACN/DMF, Technique=LC & LC/MS}—>
Protein Load Range |
|
<!— "inside defaultSpecification":{Application|MultiLine=Flexible, high-throughput format: process 1 to 96 samples|Optimized cleanup removes excess free dye, protein, and other interfering compounds|Reconstituted InstantPC dye may be stored frozen (-20°C) for up to 3 months|Small molecule size for improved chromatographic peak resolution|, Count=96, Equipment & Reagents Provided by User|MultiLine=96-well Thermocycler or two independent heat blocks, set for 90°C and 50°C. (e.g. Corning THERM-1001, 110V. THERM-1000, 230V). Note: Two GlykoPrep heaters, WS0271, can be fitted with VWR 13259-260 modular heating blocks|Acetonitrile (ACN), MS-grade (Fisher A955-4 recommended)|Formic acid, MS-grade (Fisher A117-50 recommended)|Gly-X vacuum manifold spacer (Agilent GX100)|Optional — VWR 10 kDa centrifugal filters (82031-348)|Vacuum manifold (Millipore MSVMHTS00)|Vacuum pump (Millipore WP6211560, 110 V, WP6122050, 220V, Welch WOB-L Pump 2522). Note: If you have a vacuum manifold and pump other than the Millipore model suggested, please contact us for information. Alternative vacuum manifold may be used with a GX200 spacer in place of GX100 (see FAQs)|, Kit Configuration=Sample Prep Kit, Label=InstantPC, Precision (well-to-well, day-to-day)= 95 %, Protein Load Range=1-40 µg, Recovery=> 95 %, Stability=Samples may be stored at -20°C for up to 6 months in Gly-X InstantPC eluent, or 4°C for up to 5 days in either Gly-X InstantPC eluent or after dilution with ACN/DMF, Technique=LC & LC/MS}—>
Recovery |
|
<!— "inside defaultSpecification":{Application|MultiLine=Flexible, high-throughput format: process 1 to 96 samples|Optimized cleanup removes excess free dye, protein, and other interfering compounds|Reconstituted InstantPC dye may be stored frozen (-20°C) for up to 3 months|Small molecule size for improved chromatographic peak resolution|, Count=96, Equipment & Reagents Provided by User|MultiLine=96-well Thermocycler or two independent heat blocks, set for 90°C and 50°C. (e.g. Corning THERM-1001, 110V. THERM-1000, 230V). Note: Two GlykoPrep heaters, WS0271, can be fitted with VWR 13259-260 modular heating blocks|Acetonitrile (ACN), MS-grade (Fisher A955-4 recommended)|Formic acid, MS-grade (Fisher A117-50 recommended)|Gly-X vacuum manifold spacer (Agilent GX100)|Optional — VWR 10 kDa centrifugal filters (82031-348)|Vacuum manifold (Millipore MSVMHTS00)|Vacuum pump (Millipore WP6211560, 110 V, WP6122050, 220V, Welch WOB-L Pump 2522). Note: If you have a vacuum manifold and pump other than the Millipore model suggested, please contact us for information. Alternative vacuum manifold may be used with a GX200 spacer in place of GX100 (see FAQs)|, Kit Configuration=Sample Prep Kit, Label=InstantPC, Precision (well-to-well, day-to-day)= 95 %, Protein Load Range=1-40 µg, Recovery=> 95 %, Stability=Samples may be stored at -20°C for up to 6 months in Gly-X InstantPC eluent, or 4°C for up to 5 days in either Gly-X InstantPC eluent or after dilution with ACN/DMF, Technique=LC & LC/MS}—>
Stability |
- Samples may be stored at -20°C for up to 6 months in Gly-X InstantPC eluent, or 4°C for up to 5 days in either Gly-X InstantPC eluent or after dilution with ACN/DMF
|
<!— "inside defaultSpecification":{Application|MultiLine=Flexible, high-throughput format: process 1 to 96 samples|Optimized cleanup removes excess free dye, protein, and other interfering compounds|Reconstituted InstantPC dye may be stored frozen (-20°C) for up to 3 months|Small molecule size for improved chromatographic peak resolution|, Count=96, Equipment & Reagents Provided by User|MultiLine=96-well Thermocycler or two independent heat blocks, set for 90°C and 50°C. (e.g. Corning THERM-1001, 110V. THERM-1000, 230V). Note: Two GlykoPrep heaters, WS0271, can be fitted with VWR 13259-260 modular heating blocks|Acetonitrile (ACN), MS-grade (Fisher A955-4 recommended)|Formic acid, MS-grade (Fisher A117-50 recommended)|Gly-X vacuum manifold spacer (Agilent GX100)|Optional — VWR 10 kDa centrifugal filters (82031-348)|Vacuum manifold (Millipore MSVMHTS00)|Vacuum pump (Millipore WP6211560, 110 V, WP6122050, 220V, Welch WOB-L Pump 2522). Note: If you have a vacuum manifold and pump other than the Millipore model suggested, please contact us for information. Alternative vacuum manifold may be used with a GX200 spacer in place of GX100 (see FAQs)|, Kit Configuration=Sample Prep Kit, Label=InstantPC, Precision (well-to-well, day-to-day)= 95 %, Protein Load Range=1-40 µg, Recovery=> 95 %, Stability=Samples may be stored at -20°C for up to 6 months in Gly-X InstantPC eluent, or 4°C for up to 5 days in either Gly-X InstantPC eluent or after dilution with ACN/DMF, Technique=LC & LC/MS}—>
Technique |
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