Kinetex 1.7 µm Biphenyl 100 Å, LC Column 150 x 2.1 mm, Ea
Бренд: Phenomenex
Novel Selectivity For Tough Separations
Think high performance‚ enhanced retention‚ and the ability to go where a traditional C18 can’t. The Kinetex Biphenyl offers the high performance benefits of a core-shell particle with a unique stationary phase capable of becoming the go-to selectivity for reversed phase method development. Use Kinetex Biphenyl columns to get enhanced retention‚ higher sensitivity‚ and overall better results; especially for aromatic compounds.
Aromatic Selectivity
Compounds with aromatic ring structures offer a specific type of selectivity associated with pi-pi bond interaction. The compound’s aromaticity provides pi electrons that have the potential to interact with pi bonds, which can be found on phenyl based stationary phases. This provides a unique, orthogonal selectivity compared to a traditional C18 phase.
Aromatic Compound Comparison
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Column:
Kinetex 1.7 µm Biphenyl
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Dimension:
50 x 2.1 mm
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Part No.:
00B-4628-AN
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Mobile Phase:
A: Water with 0.1 % Formic Acid B: Acetonitrile with 0.1 % Formic Acid
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Gradient:
Time (min)
0 4 5 7
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% B
5 100 100 5
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Flow Rate:
0.5 mL/min
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Temperature:
40 ˚C
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Detection:
MS/MS (Sciex® API 4000™) (ambient)
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Injection Volume:
10 μL
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Sample:
Drugs of Abuse
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Increased Separation of Aromatic Compounds Using a Biphenyl Phase
The elution order of analytes can change depending upon the predominate selectivity characteristics of a HPLC or UHPLC column and the utilized mobile phase. Therefore, by altering the stationary phase or mobile phase conditions, we can observe a unique elution order and extend retention of more polar analytes.
Aromatic Compound Comparison
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Columns:
Kinetex 1.7 µm C18 Kinetex 1.7 µm Biphenyl
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Dimension:
50 x 2.1 mm
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Part No.:
00B-4628-AN 00B-4475-AN
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Mobile Phase:
A: Water with 0.1 % Formic Acid B: Methanol with 0.1 % Formic Acid
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Gradient:
Time (min)
0 4 4.1
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% B
10 100 10
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Flow Rate:
400 µL/min
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Temperature:
50 ˚C
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Detection:
MS/MS (S
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