Compatibility
The Kinetex C18 column is fully compatible with all UHPLC instruments, ensuring seamless integration into any lab setup. This column is designed for easy installation and optimal performance. For installation, loosen the collet, place the tool between the gold compression screw and collet for quick release, adjust the tubing to the correct port depth, and connect the Kinetex column to the end-fitting. This universal compatibility allows you to leverage the powerful sub-2 µm column technology across different systems effortlessly.
The Kinetex C18 column offers a well-balanced C18 phase that delivers the highest level of hydrophobic selectivity compared to other Kinetex phases.


Upgrade from Traditional Fully Porous to Kinetex Core-Shell
Enhance your analytical method instantly by upgrading to the Kinetex C18 column for superior performance. Take advantage of the fully scalable available particle sizes of Kinetex C18 to bring improved performance to any LC system!
Core-shell columns are best suited for the rapid and precise separation of complex drug mixtures. Improve the quality control process with Kinetex Core-Shell columns.
1.7 µm Fully Porous vs. 1.7 µm Core-Shell
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Columns:
Kinetex 1.7 µm C18
Traditional 1.7 µm C18 |
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Dimension:
50 x 2.1 mm
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Mobile Phase:
Acetonitrile / Water (50:50)
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Flow Rate:
0.6 mL/min
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Temperature:
25 °C
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Detection:
UV @ 254 nm
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Instrument:
*Waters® ACQUITY® UPLC®
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Sample:
1. Acetophenone
2. Benzene 3. Toluene 4. Naphthalene |
Fully Porous vs. Core-Shell
◊ Signal-to-noise ratio of peak 2; ∆ Based on average peak widths; *Waters, ACQUITY, and UPLC are registered trademarks of Waters Corporation. Phenomenex is not affiliated with Waters Corporation. Comparative separations may not be representative of all applications. Conditions same for both columns except where noted.
Conditions for both columns:
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Columns:
Kinetex 2.6 µm C18 100 Å
Traditional 5 µm ODS-3 100 Å |
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Dimension:
Kinetex: 150 x 4.6 mm
Traditional: 250 x 4.6 mm |
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Mobile Phase:
A: 0.1 % Phosphoric acid in Water
B: 0.1 % Phosphoric acid in Acetonitrile |
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Gradient:
5 % to 95 % B in 9 min (150 x 4.6 mm)
5 % to 95 % B in 15 min (250 x 4.6 mm) |
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Flow Rate:
1.8 mL/min
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Temperature:
50 °C
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Detection:
UV @ 215 nm (22 °C)
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Sample:
1. Procainamide
2. Acetaminophen 3. Folic acid 4. Sulfathiazole 5. Acebutolol 6. Dextromethorphan 7. Diphenhydramine 8. Propafenone 9. Amitriptyline 10. Fluoxetine 11. Naproxen 12. Diflunisal 13. Indomethacin |
Performance with Kinetex C18 on HPLC and UHPLC Systems
Quickly improve the productivity and performance of your existing methods and utilize the core-shell advantage to deliver the performance you need at backpressures the system can handle.

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Columns:
Kinetex 2.6 µm C18 (Agilent 1100*)
Kinetex 2.6 µm C18 (Agilent 1290) ZORBAX 1.8 µm SB-C18 (Agilent 1290) |
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Dimension:
100 x 4.6 mm
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Mobile Phase:
A: Water with 0.1% TFA
B: Acetonitrile with 0.1% TFA |
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Gradient:
Time (min)
0
20 |
% B
10
70 |
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Flow Rate:
1.2 mL/min
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Temperature:
Ambient
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Detection:
UV @ 210 nm
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Sample:
Mupirocin degradants
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