Selectivity for Acidic Compounds
This unique C18 phase yields increased hydrogen bonding with hydrophobic selectivity‚ resulting in improved peak shape for basic compounds and increased retention of acidic compounds.
Complementary Core-Shell C18 Phases
C18 Phases (C18 vs. XB-C18)
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Dimension:
50 x 2.1 mm
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Mobile Phase:
A: Water B: Acetonitrile
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Gradient:
Time (min)
0 6 6.01 8
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% B
20 60 20 20
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Flow Rate:
0.5 mL/min
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Temperature:
30 °C
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Detection:
UV @ 220 nm (ambient)
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Samples:
1. Estriol 2. Hydrocortisone 3. Cortisone 4. Estradiol 5. Cortisone-21-acetate 6. 21-Hydroxyprogesterone 7. Estrone 8. 17-Hydroxyprogesterone 9. Deoxycorticosterone acetate
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Manipulate Acidic and Basic Compound Retention
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Dimension:
50 x 2.1 mm
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Mobile Phase:
A: Water with 0.1% Formic Acid B: Acetonitrile with 0.1% Formic Acid
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Gradient:
Time (min)
0 0.2 4.2 4.21 5.5
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% B
5 5 95 5 5
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Flow Rate:
0.8 mL/min
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Temperature:
30 °C
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Detection:
UV @ 245 nm (ambient)
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Samples:
1. Pyridine 2. Acetaminophen 3. Pindolol 4. Quinidine 5. Sulfathiazole 6. Acebutolol 7. Benzyl alcohol 8. Chlorpheniramine 9. Phenol 10. Triprolidine 11. Nortriptyline 12. Prednisolone 13. 3-Methyl, 4-nitrobenzoic acid 14. 2-Hydroxy, 5-methylbenzaldehyde 15. Diflunisal 16. Hexanophenone
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