Selectivity for Acidic Compounds
This unique C18 phase yields increased hydrogen bonding with hydrophobic selectivity‚ resulting in improved peak shape for basic compounds and increased retention of acidic compounds.

C18 Phases (C18 vs. XB-C18)
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Dimension:
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Mobile Phase:
A: Water B: Acetonitrile
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Gradient:
Time (min)
0 6 6.01 8
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% B
20 60 20 20
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Flow Rate:
0.5 mL/min
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Temperature:
30 °C
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Detection:
UV @ 220 nm (ambient)
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Samples:
1. Estriol 2. Hydrocortisone 3. Cortisone 4. Estradiol 5. Cortisone-21-acetate 6. 21-Hydroxyprogesterone 7. Estrone 8. 17-Hydroxyprogesterone 9. Deoxycorticosterone acetate
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Manipulate Acidic and Basic Compound Retention
Find the right phase selectivity for your application by investigating the diverse selectivity availability of the Kinetex Core Shell HPLC and UHPLC columns. Where reliable performance meets unique selectivity.
Conditions for all columns:
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Dimension:
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Mobile Phase:
A: Water with 0.1% Formic Acid B: Acetonitrile with 0.1% Formic Acid
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Gradient:
Time (min)
0 0.2 4.2 4.21 5.5
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% B
5 5 95 5 5
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Flow Rate:
0.8 mL/min
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Temperature:
30 °C
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Detection:
UV @ 245 nm (ambient)
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Samples:
1. Pyridine 2. Acetaminophen 3. Pindolol 4. Quinidine 5. Sulfathiazole 6. Acebutolol 7. Benzyl alcohol 8. Chlorpheniramine 9. Phenol 10. Triprolidine 11. Nortriptyline 12. Prednisolone 13. 3-Methyl, 4-nitrobenzoic acid 14. 2-Hydroxy, 5-methylbenzaldehyde 15. Diflunisal 16. Hexanophenone
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