Luna 5 µm PFP(2) 100 Å, LC Column 150 x 3 mm, Ea

Ultra-pure, metal-free silica (99.99 % purity) is the backbone of all Luna material. The resulting high quality particles have a surface smoothness, pore structure, and pore consistency to ensure a more uniform particle shape and greater reproducibility.

Agilent Technologies® ZORBAX® 5 µm SB-C18

Waters® Symmetry® 5 µm C18

Phenomenex Luna 5 µm C18

Luna® PFP(2) columns provide unique selectivity for highly polar compounds, complex natural products, isomers and other closely related compounds. This is achieved by using a propyl linked pentafluorophenyl, which provides multiple retention mechanisms unique to typical reversed phase medias.

1. Hydrogen Bonding
2. Dipole-Dipole Interactions
3. Aromatic and π-π Interactions
4. Hydrophobic

A typical alkyl phase (C18‚ C8) achieves selectivity through only 1 mechanism of interaction.

Positional changes on an analyte of interest may effect the compound’s dipole moment. This change can be readily seen by the way the highly electronegative fluorine (F) atoms and other retention mechanisms of the Luna PFP(2) are able to separate positional isomers.

The high efficiencies and bonded phase surface coverage provide for sharp peaks The C 8 columns are generally better hydrogen bond acceptors, and better for acidic compounds.

Column: Luna 3 µm PFP(2) Luna 3 µm C18(2)

Dimensions 150 x 4.6 mm

Part No.: 00F-4447-E0 00F-4251-E0

Mobile Phase: Water/Methanol (50:50)

Flow Rate: 1 mL/min

Temperature: 22 °C

Detection: UV @ 254 nm

Sample: 1. o-Methylacetophenone 2. m-Methylacetophenone 3. p-Methylacetophenone