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A C18 With a Positive Twist
Luna Omega PS C18 has been fine tuned and manufactured by Phenomenex to provide a mixed selectivity that is highly useful for method development involving either combinations of polar and nonpolar compounds, or just one single compound class with small changes in functional groups.
Enhanced Selectivity and Polar Retention
With its positive surface (PS) the Luna Omega PS C18 provides valuable increase in retention of acids through ionic/polar interactions. In addition, the C18 ligand also stimulates hydrophobic retention that greatly promotes increased resolution between analytes of interest.
MMA and Succinic Acid
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Columns:
Luna Omega 1.6 μm PS C18 ACE Excel 2 μm C18-AR
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Dimension:
50 x 2.1 mm
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Mobile Phase:
A: Water with 0.1 % Formic Acid B: Acetonitrile with 0.1 % Formic Acid
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Gradient:
Time (min)
0 5 5.1 7
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% B
0 50 0 0
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Flow Rate:
0.5 mL/min
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Temperature:
22 °C
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Detection:
MS/MS (SCIEX® API 4000™)
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Samples:
1. Succinic acid 2. MMA
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Think Outside the Traditional C18 Selectivity
Traditional C18 phases may not always be the best option. A HPLC and UHPLC column that has both polar and hydrophobic versatility allows for great method development flexibility.
Acids/Bases/Neutrals
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Columns:
Luna Omega 1.6 μm PS C18 ACQUITY BEH 1.7 μm C18
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Dimension:
50 x 2.1 mm
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Mobile Phase:
A: Water with 0.1 % Formic Acid B: Acetonitrile with 0.1 % Formic Acid
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Gradient:
Time (min)
0 5 5.01 8
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% B
5 95 5 5
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Flow Rate:
0.4 mL/min
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Temperature:
22 °C
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Detection:
UV @ 254 nm
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Samples:
1. Uracil 2. Pindolol 3. Chlorpheniramine 4. Nortriptyline 5. 3-Methyl-4-nitrobenzoic acid 6. 5-Methyl salicylaldehyde 7. Hexanophenone
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Better Peak Shape for Bases
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