Phenogel 5 µm 100 Å, LC Column 600 x 7.8 mm, Ea
Бренд: Phenomenex
Applications on Phenogel Fixed Pore 100Å Non-Aqueous GPC/SEC Columns
Polyethylene Glycol 330
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Columns:
Phenogel 5 µm 50 Å, 100 Å, 500 Å
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Dimension:
300 x 7.8 mm
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Part No.:
00H-0441-K0 (50 Å) 00H-0442-K0 (100 Å) 00H-0443-K0 (500 Å)
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Solvent:
THF
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Flow Rate:
1.0 mL/min
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Temperature:
Ambient
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Detection:
Differential Refractometer
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Injection Volume:
100 µL 0.25% w/v
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Sample:
1. dp7 2. dp6 3. dp5 4. dp4 5. dp3 6. dp2 7. dp1
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546 MW 458 MW 370 MW 282 MW 194 MW 106 MW 62 MW
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Closely Related Hydrocarbons
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Columns:
Phenogel 5 µm 50 Å, 100 Å, 500 Å
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Dimension:
300 x 7.8 mm
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Part No.:
00H-0441-K0 (50 Å) 00H-0442-K0 (100 Å) 00H-0443-K0 (500 Å)
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Solvent:
THF
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Flow Rate:
1.0 mL/min
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Temperature:
Ambient
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Detection:
Differential Refractometer
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Injection Volume:
100 µL 0.25% w/v
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Sample:
1. C40 2. C32 3. C24 4. C20 5. C16 6. C13
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562 MW 450 MW 338 MW 482 MW 226 MW 684 MW
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Isoprenes from In Vitro Translation on Products
The columns were used in tandem to characterize isoprene chain lengths removed from labeled in vitro translation products or cell proteins. The isoprenoids were removed by treatment with Raney nickel and extracted into pentane. The pentane extractable material was hydrogenated over platinum catalyst and injected onto the column. Fractions were collected at 0.5 minute intervals and radioactivity was monitored by liquid scintillation. These saturated hydrocarbon chains were characterized by comparing radioactive peaks to standard retention times.
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Columns:
Phenogel 5 µm 50 Å, 100 Å in series
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Dimension:
300 x 7.8 mm
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Part No.:
00H-0441-K0 (50 Å) 00H-0442-K0 (100 Å)
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Solvent:
THF
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Flow Rate:
0.25 mL/min
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Temperature:
Ambient |
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Detection:
Differential Refractometer |
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Sample:
1. Squalene C30 (2, 6, 10, 15, 19, 23-Hexamethyltetracosane) 2. Phytane C20 (2, 6, 10, 14-Tetramethylhexadane) 3. Farnesne C15 (2, 6, 10-Trimethyldodecane) |
Environmental and Biomonitoring
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