The ProteinWorks eXpress Direct Digest Kit simplifies and accelerates the direct digestion of whole, non-immunopurified plasma & serum samples for LC-MS protein quantification via the surrogate peptide approach. The kit includes pre-measured, lot-traceable reagents, a flexible 96-tube sample collection module, and optimized protocols, offering scientists a standardized, proven route to fast, accurate and precise protein quantification for protein bioanalysis.
The ProteinWorks eXpress Direct Digest Kit contains:
(96) Denaturant* Cluster Tubes, Cap Mat & Rack for high protein content samples (1.0 — 5.25 mg total protein per sample)
(12) 8-Strip Caps for Cluster tubes
(1) Digestion Buffer
(1) Trypsin Dissolving Agent
(1) Digestion Inactivation Agent
(3) Reduction Agent vials. Each vial is enough for 32 samples
(3) Alkylation Agent vials. Each vial is enough for 32 samples
(1) Trypsin for high protein content samples (1.0 — 5.25 mg total protein per sample)
*The denaturant is RapiGest™ SF, an enzyme friendly detergent used to denature proteins prior to digestion. For more information on RapiGest SF, see 720000468en.
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Number of Analysis
96
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Type
Sample Preparation Workflow
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Packages per kit
2
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Separation Mode
RPLC
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Detection Method
MS/MS
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Storage Condition
Refer to the storage recommendations of each sub-package
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System Type
LC-MS
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Shelf Life
Refer to the storage recommendations of each sub-package
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UNSPSC
41116104
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Application
Protein, Peptide
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Brand
ProteinWorks
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Product Type
Application Kits
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Units per Package
1 pk