SialoCapper™-ID Kit reliably stabilizes sialic acid residues, enabling linkage isomer discrimination.
Our technology prevents the decomposition of sialic acid residues and ionization suppression. Furthermore, makes it possible to discriminate the linkage isomers of sialic acids by mass spectrometry.
Principle of MALDI-TOFMS
Various types of mass spectrometers can be used for measuring glycans derivatized with the SialoCapper-ID Kit
The SALSA method involved in the SialoCapper-ID Kit was developed by Shimadzu corporation and improved in cooperation with Prof. Jun-Ichi Furukawa and Dr. Hisatoshi Hanamatsu at Hokkaido University.
Kit Contents |
Reagent A (1 bottle), B(10 tubes), C(1 bottle) |
---|---|
Number of samples |
Sufficient for 10 experiments/kit |
Derivatization Targets |
Released N-linked glycans (including labeled forms) |
Storage Temperature |
2-8 ºC |
-For solid-phase reaction, glycan purification beads are required but not included in SialoCapper-ID Kit.
Recommended:Sumitomo Bakelite BlotGlyco® BS-45414 (for 10 samples)、BS-45415 (for 50 samples) 、BS-45407 (for 100 samples)
-For liquid-phase reaction, HILIC mode glycan purification columns or tips are required but not included in SialoCapper-ID Kit.
Downloads
Download the latest brochure.
SialoCapper-ID Kit [ PDF / 4.03MB ]