SP6 RNA Polymerase

SP6 RNA Polymerase is a DNA-dependent RNA polymerase with high sequence specificity for SP6 promoter sequences. SP6 RNA polymerase synthesizes RNA 5′ to 3′ and incorporates  35S, 32P and 33P ribonucleotides. This enzyme is isolated from an overproducing recombinant E. coli clone.SDS-polyacrylamide gel electrophoresis analysis of the SP6 RNA Polymerase assures 99% purity.Unit definition: One unit will catalyze the incorporation of 1 nmole of nucleoside triphosphate into an acid insoluble form in 60 minutes at 37°C.Reaction Conditions: 40 mM Tris-HCl (pH 8.0), 8 mM MgCl2, 50 mM NaCl, 2 mM spermidine, 30 mM DTT, 400 µM of each rNTP, 1 µg DNA template and 20 units enzyme in a 25-µl volume. Incubate 60 minutes at 37°C.