Strata-X-A 33 µm Polymeric Strong Anion, 10 mg / well, 96-Well Plates, 2/Pk
Бренд: Phenomenex
3 Mechanism of Retention
Strong Anion-Exchange
π-π Bonding
Hydrophobic Interaction
Available Formats
96-Well-Plate
96-Well-Plate
1, 3, and 6mL Tubes
Giga Tubes (12-150mL Tubes)
Starting Method
For Acids with pKa > 2
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Column:
1 mL Methanol
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Equilibrate:
1 mL Water, pH 6-7
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Load:
Diluted Sample pH 6-7
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Wash 1:
1 mL 25 mM Ammonium Acetate Buffered, pH 6-7
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Wash 2:
1 mL Methanol (collect this fraction to analyze Neutral/Bases)
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Elute Acid:
2x 500 µL 5 % Formic Acid in Methanol
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Based on 30 mg/1 mL sorbent mass. The above is a convenient starting point for SPE
method development, further optimization may be required to tailor the method for your
specific extraction needs.
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