Strata-X-Drug B 33 µm Polymeric Strong Cation, 10 mg / 1 mL, Tubes, 100/Pk
Бренд: Phenomenex
General Extraction
- Extract over 11 drugs using the same sorbent
- Consolidate methods; 3 methods is all you’ll need to extract 6 different drug classes
- Time and solvent savings; no conditioning required
- Consistent, sensitive, and high recoveries of SAMHSA regulated drugs
Retention Mechanism
Strata-X Drug B Protocol
Strata-X Drug B Plus Protocol
Starting Method
General Urine Pre-treatment Recommendation
- Combine urine sample with appropriate buffer.
- Add β-Glucuronidase enzyme according to manufacturer’s recommendation (If using Strata-X-Drug B Plus, these steps can be performed in-well).
- Incubate for specified amount of time (If using Strata-X-Drug B Plus, pulse vacuum to load solution onto sorbent).
- Follow appropriate SPE protocol based on analytes of interest.
Choose Your Analytes
| Opiates, 6-MAM, PCP, Amphetamines, Propoxyphene, Healthcare Opiates, and Methadone* | Marijuana Metabolites | Cocaine Metabolites | |
|---|---|---|---|
| Condition | Not Required | Not Required | Not Required |
| Load | Pre-treated urine sample | Pre-treated urine sample | Pre-treated urine sample |
| Wash 1 | 600 µL of 100 mM Sodium acetate buffer (pH 5.0) | 600 µL of 100 mM Sodium acetate buffer (pH 5.0) | 600 µL of 0.1 N Hydrochloric acid |
| Wash 2 | 600 µL Methanol | 600 µL of Acetonitrile/100 mM Sodium acetate buffer (pH 5.0) (30:70) | 600 µL Methanol |
| Dry | 10 minutes under full vacuum | 15 minutes under full vacuum | 10 minutes under full vacuum |
| Elute | 2x 300 µL of Ethyl acetate/Isopropanol/ Ammonium hydroxide (70:20:10) | 2x 300 µL of Ethyl acetate/Isopropanol (85:15) | 2x 300 µL of Ethyl acetate/Isopropanol/ Ammonium hydroxide (70:20:10) |
* Opiates, 6-MAM, PCP, Amphetamines, Propoxyphene, Healthcare Opiates, and Methadone can be extracted simultaneously or separately using the same SPE methodology. Methods are written for 30 mg/well Strata-X-Drug B 96-well plate; however they can be scaled to accommodate smaller or larger sample sizes and sorbent masses.
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